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Product Info Summary
| SKU: | PA1809 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Daxx Antibody Picoband®
SKU/Catalog Number
PA1809
BA0827-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Daxx Antibody catalog # PA1809. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Daxx Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1809)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Daxx, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1809 is reactive to DAXX in Human, Mouse, Rat
Observed Molecular Weight
115 kDa
Calculated molecular weight
81.4 kDa
Background of DAXX
DAXX (death-domain associated protein) also known as DAP6 (Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. Human DAXX encodes a 740-amino acid polypeptide containing a nuclear localization signal. Functional analyses by Yang et al. (1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis. The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas. The DAXX gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes. MSP58 overexpression relieved DAXX-mediated transcriptional repression. Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of DAXX interacts with the C terminus of DMAP. Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1809 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Positive Control
WB: human Hela whole cell, human Ramos whole cell, human MCF-7 whole cell, human Jurkat whole cell, rat testis tissue, mouse testis tissue
ICC/IF: U2OS cell
Validation Images & Assay Conditions
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Western blot analysis of DAXX using anti-DAXX antibody (PA1809).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Ramos whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAXX antigen affinity purified polyclonal antibody (Catalog # PA1809) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAXX at approximately 115 kDa. The expected band size for DAXX is at 81 kDa.
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IF analysis of DAXX using anti-DAXX antibody (PA1809) and anti-Beta Tubulin antibody (M01857-3).
DAXX was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DAXX Antibody (PA1809) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Daxx Antibody Picoband® (PA1809)
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4 Customer Q&As for Anti-Daxx Antibody Picoband®
Question
Is there a BSA free version of anti-Daxx antibody PA1809 available?
J. Roberts
Verified customer
Asked: 2019-10-09
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Daxx antibody PA1809 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Daxx antibody PA1809. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-02
Question
I was wanting to use to test anti-Daxx antibody PA1809 on human liver for research purposes, then I may be interested in using anti-Daxx antibody PA1809 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
The products we sell, including anti-Daxx antibody PA1809, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-12
Question
Will PA1809 anti-Daxx antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Moore
Verified customer
Asked: 2019-04-02
Answer
As indicated on the product datasheet, PA1809 anti-Daxx antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-02
