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- Table of Contents
TNFRSF10A antibody (A02152) performs well in IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IF analysis of ATG14L using anti-ATG14L antibody (A02152).
ATG14L was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATG14L Antibody (A02152) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ATG14L was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATG14L Antibody (A02152) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-DR4/TNFRSF10A Antibody Picoband®
TNFRSF10A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, ICC, WB |
Tested Applications
TNFRSF10A antibody was positively detected by WB in:
human A549 whole cell, human Hacat whole cell, human RT4 whole cell, human K562 whole cell, rat spleen tissue, rat lung tissue, mouse spleen tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TNFRSF10A antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence
5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-DR4/TNFRSF10A Antibody Picoband®
View all TNFRSF10A Antibodies
View all TNFRSF10A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human DR4.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
50.1 kDa
Background of TNFRSF10A
TNFRSF10A (Tumor Necrosis Factor Receptor Subfamily Member 10A), also known as APO2, DR4 or TRAILR1, is a protein that in humans is encoded by the TNFRSF10A gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
Cite This Product
Anti-DR4/TNFRSF10A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02152)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
91%
High Homology
Rat
36%
Low homology
Mouse
27%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TNFRSF10A antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TNFRSF10A antibody
Publications
Application:
Species:
miR-942 decreases TRAIL-induced apoptosis through ISG12a downregulation and is regulated by AKT
Published: 2014-06-06
Journal: Oncotarget
Application: WB
Impact Factor: 6.63
Species: Human
PMID: 24970806
The Novel Fusion Proteins, GnRH-p53 and GnRHIII-p53, Expression and Their Anti-Tumor Effect
Published: 2013-11-04
Journal: PLoS One
Application: IF
Impact Factor: 3.73
Species: Human
PMID: 24223939
DJ-1 inhibits TRAIL-induced apoptosis by blocking pro-caspase-8 recruitment to FADD
Published: 2011-07-25
Journal: ONCOGENE
Application: WB
Impact Factor: 7.41
Species: Human
PMID: 21785459
Plumbagin enhances TRAIL-mediated apoptosis through up-regulation of death receptor in human melanoma A375 cells
Published: 2010-08-17
Journal: Journal of Huazhong University of Science and Technology-Medical Sciences
Application: WB
Impact Factor: 0.31
Species: Human
PMID: 20714870
The Occurrence and Regional Distribution of DR4 on Herniated Disc Cells: A Potential Apoptosis Pathway in Lumbar Intervertebral Disc
Published: 2008-02-15
Journal: SPINE
Application: IHC
Impact Factor: 2.50
Species: Human
PMID: 18277875
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Product Promise
We promise Anti-DR4/TNFRSF10A Antibody Picoband® performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02152
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026