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CD69 antibody (A00529-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of human PBMC cells using anti-CD69 antibody (A00529-2).
Overlay histogram showing human PBMC cells stained with A00529-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD69 Antibody (A00529-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing human PBMC cells stained with A00529-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD69 Antibody (A00529-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CD69 Antibody Picoband®
CD69 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
CD69 antibody was positively detected by WB in:
human K562 whole cell, human HL-60 whole cell, human Raji whole cell, mouse SP2/0 whole cell, rat thymus tissue, mouse thymus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CD69 antibody was positively detected by IHC in:
human B lymphocytic tumor tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CD69 antibody was positively detected by ICC/IF in:
K562 cell
CD69 antibody was positively detected by FC in:
human PBMC cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
4μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
FC
Human
Tested
Tested
Recommend
Recommend
Tested
Mouse
Tested
Recommend
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD69 Antibody Picoband®
View all CD69 Antibodies
View all CD69 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD69 recombinant protein (Position: T101-E148).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
22.6 kDa
Background of CD69
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets.
Cite This Product
Anti-CD69 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00529-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
92%
High Homology
Mouse
69%
Low homology
Cow
67%
Low homology
Rat
65%
Low homology
Sheep
65%
Low homology
Rabbit
63%
Low homology
Dog
63%
Low homology
Drosophila
23%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CD69 antibody
Immunohistochemistry (IHC) protocol for CD69 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CD69 antibody
Flow Cytometry (FC) protocol for CD69 antibody
Publications
Application:
Species:
Capsid inclusion in a CHIKV mRNA vaccine impairs adaptive immune responses
Published: 2026-08-03
Journal: Cell Reports Medicine
Application: IF
Impact Factor: 14.00
Species: Monkey
PMID: 42546698
Methylprednisolone attenuates tendon adhesion via modulating the eIF3a-TGF-β1 Axis in tenocytes and CCS-ROS-NLRP3 Axis in macrophages
Published: 2026-03-03
Journal: CHEMICAL ENGINEERING JOURNAL
Application: IF
Impact Factor: 13.20
Species: Rat
The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis
Published: 2023-03-07
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IHC
Impact Factor: 8.20
Species: Mouse
PMID: 36889619
Review
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Product Promise
We promise Anti-CD69 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00529-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026