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- Table of Contents
Adgre1 antibody (A08751) performs well in IF, IHC and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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IF analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-F4/80/Adgre1 Antibody
Adgre1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IF, IHC |
Tested Applications
Adgre1 antibody was positively detected by IHC in:
rat liver tissue, mouse liver tissue, mouse spleen tissue, mouse kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Adgre1 antibody was positively detected by IF in:
mouse liver tissue, mouse spleen tissue, rat liver tissue
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 µg/ml
Mouse, Rat
Immunofluorescence
5 µg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-F4/80/Adgre1 Antibody
View all ADGRE1 Antibodies
View all ADGRE1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml stabilizing protein and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse F4/80/Adgre1 recombinant protein (Position: G27-S230).
Cross-Reactivity
No cross-reactivity with other proteins.
Calculated Molecular Weight
102.1 kDa
Background of Adgre1
Predicted to enable G protein-coupled receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway. Predicted to act upstream of or within G protein-coupled receptor signaling pathway and adaptive immune response. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system; central nervous system; genitourinary system; hemolymphoid system; and intestine. Orthologous to human ADGRE1 (adhesion G protein-coupled receptor E1).
Cite This Product
Anti-F4/80/Adgre1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08751)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
87%
Moderate Homology
Dog
64%
Low homology
Human
60%
Low homology
Sheep
55%
Low homology
Cow
51%
Low homology
Monkey
30%
Low homology
Zebrafish
16%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for Adgre1 antibody
Immunofluorescence (IF) protocol for Adgre1 antibody
Publications
Application:
Species:
Mendelian randomization–inspired targeted polyphenol nanoplatform for synergistic osteoarthritis therapy
Published: 2026-03-15
Journal: Acta Pharmaceutica Sinica B
Application: IF
Impact Factor: 14.60
Species: Mouse
Synergistic proanthocyanidin-copper oxygen-generating microneedle enhances anti-inflammatory activity in dental pulp stem cells and macrophage efferocytosis
Published: 2026-03-10
Journal: BMC Oral Health
Application: IF
Impact Factor: 3.10
Species: Mouse
PMID: 41808121
Platycodin D alleviates idiopathic pulmonary fibrosis by targeting PPP2R1A and regulating the PP2A-mediated PI3K/Akt pathway to inhibit fibroblast transdifferentiation
Published: 2026-02-21
Journal: PHYTOMEDICINE
Application: mIF
Impact Factor: 8.30
Species: Mouse
PMID: 41759424
Photobiomodulation mitigates chondrocyte catabolism in osteoarthritis by modulating macrophage M1 polarization through the IL-6/JAK/STAT pathway
Published: 2025-12-24
Journal: Journal of Orthopaedic Surgery and Research
Application: IF
Impact Factor: 2.80
Species: Mouse
PMID: 41444971
GLDC attenuates liver ischemia-reperfusion injury by inhibiting macrophage recruitment and activation via PTBP1/P2RY6
Published: 2025-07-03
Journal: CELLULAR SIGNALLING
Application: WB
Impact Factor: 3.70
Species: Rat
PMID: 40617371
Bioengineered platelet nanoplatform enables renal-targeted dexamethasone delivery for chronic nephritis therapy with dual anti-inflammatory/anti-fibrotic effects and minimized systemic toxicity
Published: 2025-06-07
Journal: Bioactive Materials
Application: IHC,IF
Impact Factor: 20.30
Species: Mouse
PMID: 40535704
LXRα agonists ameliorates acute rejection after liver transplantation via ABCA1/MAPK and PI3K/AKT/mTOR signaling axis in macrophages
Published: 2025-03-14
Journal: MOLECULAR MEDICINE
Application: FC
Impact Factor: 6.40
Species: Rat
PMID: 40087552
A comprehensive single-cell RNA transcriptomic analysis identifies a unique SPP1+ macrophages subgroup in aging skeletal muscle
Published: 2024-08-06
Journal: Scientific Reports
Application: IF
Impact Factor: 3.80
Species: Mouse
PMID: 39103421
High-fructose corn syrup promotes proinflammatory Macrophage activation via ROS-mediated NF-κB signaling and exacerbates colitis in mice
Published: 2022-05-06
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IHC
Impact Factor: 5.71
Species: Mouse
PMID: 35533555
Combination of pseudoephedrine and emodin ameliorates LPS-induced acute lung injury by regulating macrophage M1/M2 polarization through the VIP/cAMP/PKA pathway
Published: 2022-02-05
Journal: Chinese Medicine
Application: IF,IHC
Impact Factor: 4.55
Species: Rat
PMID: 35123524
Autologous bionic tissue for inguinal hernia repair
Published: 2020-02-24
Journal: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Application: IHC
Impact Factor: 3.53
Species: Rabbit
PMID: 32090432
CD89-mediated recruitment of macrophages via a bispecific antibody enhances anti-tumor efficacy
Published: 2017-10-12
Journal: OncoImmunology
Application: IF
Impact Factor: 7.72
Species: Human
PMID: 29296544
Simultaneous exposure to FcγR and FcαR on monocytes and macrophages enhances antitumor activity in vivo
Published: 2017-04-10
Journal: Oncotarget
Application: IF
Impact Factor: 5.17
Species: Mouse
PMID: 28454118
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Product Promise
We promise Anti-F4/80/Adgre1 Antibody performs excellently in relevant applications for IF, IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A08751
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026