Anti-F4/80/Adgre1 Antibody

IHC analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IF analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IF analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IF analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Pseudoephedrine + emodin inhibited (M1) macrophage and activated (M2) macrophage in LPS‐induced rats lung tissue. A F4/80 expression was determined using immunohistochemistry (n = 3). B F4/80 positive score was determined using ImageJ: negative (0), low positive (1+), positive (2+), high positive (3+). C – F CD86, CD206 expression was determined using immunofluorescence (n = 5). Data are expressed as mean ± S.D. ## p < 0.01, ### p < 0.001 vs. control group. **p < 0.01, ***p < 0.001 vs. LPS alone group. +++ p < 0.001 vs. combined treatment group (20 + 40 mg/kg)
Index in PubMed under a CC BY license. PMID: 35123524

ADCP assays with BMDMs. In vitro assay to determine elimination of Raji cells by macrophages. (A) BMDMs from WT and FcαRI Tg C57BL/6 mice were incubated with CFSE-labeled Raji cells at an E:T ratio of 5:1 in the presence of 10 μg/mL anti-CD20 antibodies. After a 4-h incubation, cells were transferred to a new tube and visualized via confocal microscopy. Representative images of ADCP mediated by anti-CD20 antibodies are shown. (B) BMDMs were labeled with APC-F4/80 antibodies and cocultured with CFSE-labeled Raji cells in the presence of the indicated antibodies. Phagocytosis of Raji cells was analyzed by FACS and quantified as the percentage of double-positive cells relative to total CFSE-positive cells and F4/80+cells. ** P < 0.01 using unpaired two-tailed t tests.
Index in PubMed under a CC BY license. PMID: 28454118

Effects of co-adjustment of TO901317 and tacrolimus on AR following liver transplantation in rats. A – D Hepatic pathological changes and apoptosis following co-adjustment with TO901317 and tacrolimus during AR (magnification, ×200; scale bar = 100 μm) (The area indicated by the red arrow shows inflammatory cell infiltration in the portal area and hepatic sinusoids, cholangitis and venulitis, as well as hepatic parenchymal cell damage). E , F The percentages of M1-type polarized macrophages in different groups were detected by flow cytometry, macrophages were separated with FITC-labeled F4/80, and M1-type polarization was separated with PE-labeled CD86. G ALT and AST levels in rat serum. H Survival rate of rats. n = 6 for each group. I Levels of TNF-α IL-6 and IL-1β in serum of different groups. Differences among groups were assessed by one-way ANOVA. n = 6, ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001
Index in PubMed under a CC BY license. PMID: 40087552

LXRα improves AR after liver transplantation by activating PI3K/AKT/mTOR pathway to regulate macrophage polarization. A , B Protein expression levels of PI3K/AKT/mTOR signaling pathway factors. C , D Detection of the proportion of M1 polarization by flow cytometry, macrophages were separated with FITC-labeled F4/80, and M1-type polarization was separated with PE-labeled CD86. E , F Pathological changes in the liver following treatment with TO901317 or LY294002 during AR (magnification, ×200; scale bar = 100 μm) (The area indicated by the red arrow shows inflammatory cell infiltration in the portal area and hepatic sinusoids, cholangitis and venulitis, as well as hepatic parenchymal cell damage). One-way ANOVA was used to determine the statistical differences between groups. n = 6, *P < 0.05, **P < 0.01, ***P < 0.001
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Effects of ABCA1 in vivo. A , B Pathological changes in the liver following treatment with TO901317 and ABCA1 shRNA during AR (magnification, ×200/400; scale bar = 100 μm) (The area indicated by the red arrow shows inflammatory cell infiltration in the portal area and hepatic sinusoids, cholangitis and venulitis, as well as hepatic parenchymal cell damage). C , D Hepatic apoptosis following treatment with TO901317 and ABCA1 shRNA during AR (magnification, ×200; scale bar = 100 mm). E , F Protein expression levels of LXRα/ABCA1/MAPK and mTOR signaling pathway factors. E Detection of the proportion of M1 polarization by flow cytometry, macrophages were separated with FITC-labeled F4/80, and M1-type polarization was separated with PE-labeled CD86. One-way ANOVA was used to determine the statistical differences between groups. n = 6, ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001
Index in PubMed under a CC BY license. PMID: 40087552