Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband™

15-Lipoxygenase 1 antibody

Boster Bio Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband™ catalog # PB9073. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9073
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband™

View all 15-Lipoxygenase 1 Antibodies

SKU/Catalog Number

PB9073

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband™ catalog # PB9073. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9073)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9073 is reactive to ALOX15 in Human, Mouse, Rat

Applications

PB9073 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

74.804kDa

Background of 15-Lipoxygenase 1

Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene. This gene is mapped to 17p13.2. It is found that ALOX15 is a mutator gene. ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in directionally opposite manners.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For ALOX15 (Source: Uniprot.org, NCBI)

Gene Name

ALOX15

Full Name

Arachidonate 15-lipoxygenase

Weight

74.804kDa

Superfamily

lipoxygenase family

Alternative Names

15-lipooxygenase-1; 15-LOX-1; 15LOX-1; arachidonate 15-lipoxygenase; Arachidonate omega-6 lipoxygenase; EC 1.13.11; EC 1.13.11.33; LOG15,15-LOX ALOX15 12-LOX, 15-LOX, 15-LOX-1, LOG15 arachidonate 15-lipoxygenase polyunsaturated fatty acid lipoxygenase ALOX15|12/15-lipoxygenase|15-LOX type 1|15-lipooxygenase-1|15-lipoxygenase type 1|arachidonate 12-lipoxygenase, leukocyte-type|arachidonate omega-6 lipoxygenase|hepoxilin A3 synthase Alox15|linoleate 13S-lipoxygenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALOX15, check out the ALOX15 Infographic

ALOX15 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALOX15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for PB9073

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

6 Customer Q&As for Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband™

Question

We are currently using anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

J. Parker

Verified customer

Asked: 2020-03-04

Answer

The anti-15 Lipoxygenase 1/ALOX15 antibody (PB9073) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-04

Question

See below the WB image, lot number and protocol we used for tongue using anti-15 Lipoxygenase 1/ALOX15 antibody PB9073. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-07

Question

Would anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 work for WB with tongue?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

According to the expression profile of tongue, ALOX15 is highly expressed in tongue. So, it is likely that anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 will work for WB with tongue.

Boster Scientific Support

Answered: 2019-08-07

Question

Would anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 work on monkey IHC-P with brain lung?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

Our lab technicians have not tested anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain lung in IHC-P, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-24

Question

Is this PB9073 anti-15 Lipoxygenase 1/ALOX15 antibody reactive to the isotypes of ALOX15?

Verified Customer

Verified customer

Asked: 2019-02-08

Answer

The immunogen of PB9073 anti-15 Lipoxygenase 1/ALOX15 antibody is E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-08

Question

Is a blocking peptide available for product anti-15 Lipoxygenase 1/ALOX15 antibody (PB9073)?

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

We do provide the blocking peptide for product anti-15 Lipoxygenase 1/ALOX15 antibody (PB9073). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-04-19

Size

Conjugation

Total: $370

SKU:PB9073

In stock, 2 left.

Order within 2 hours and 3 minutes to receive by Wed Mar 20

Get A Quote
Eddy test
In stock
Order Product
PB9073
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.