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- Table of Contents
FASN antibody (PB9865) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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IF analysis of FASN using anti-FASN antibody (PB9865).
FASN was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FASN Antibody (PB9865) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
FASN was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FASN Antibody (PB9865) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Fatty Acid Synthase/FASN Antibody Picoband®
FASN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
FASN antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, human hepatocellular carcinoma tumor tissue (HCCT), human 293T whole cell, human MCF-7 whole cell, human RT4 whole cell, human A549 whole cell, rat liver tissue, mouse lung tissue, mouse liver tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FASN antibody was positively detected by IHC in:
human intestinal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FASN antibody was positively detected by ICC/IF in:
T47D cell
FASN antibody was positively detected by IF in:
human ovarian cancer tissue, human rectal cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
5 μg/ml
Immunofluorescence
5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Mouse
Tested
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Fatty Acid Synthase/FASN Antibody Picoband®
View all FASN Antibodies
View all FASN Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
273 kDa
Calculated Molecular Weight
273.4 kDa
Background of FASN
Fatty acid synthase (FAS) is an enzyme that in humans is encoded by the FASN gene. It is mapped to 17q25. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Cite This Product
Anti-Fatty Acid Synthase/FASN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9865)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
96%
High Homology
Rat
91%
High Homology
Mouse
90%
High Homology
Dog
88%
Moderate Homology
Sheep
88%
Moderate Homology
Zebrafish
76%
Moderate Homology
Cow
65%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FASN antibody
Immunohistochemistry (IHC) protocol for FASN antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FASN antibody
Immunofluorescence (IF) protocol for FASN antibody
Publications
Application:
Species:
Multi-omics integration reveals the ameliorative effects and underlying mechanisms of Astragalus membranaceus (Huangqi)-Fuzhuan brick tea on nonalcoholic fatty liver disease
Published: 2026-03-31
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 41933745
The Artemisia argyi oil reduces high-fat diet-induced obesity by enhancing thermogenesis in brown adipose tissue
Published: 2025-12-13
Journal: npj Science of Food
Application: WB
Impact Factor: 7.80
Species: Mouse
PMID: 41390517
Camel-Milk-Derived Extracellular Vesicles Mitigate High-Fat-Diet-Induced Obesity by Enhancing Thermogenesis and Modulating Lipid Metabolism in Mice
Published: 2025-08-14
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB
Impact Factor: 6.20
Species: Mouse
PMID: 40810347
GCN2 promotes fatty acid metabolism through the activation of PI3K/AKT signaling in gastric cancer
Published: 2025-07-10
Journal: CELLULAR SIGNALLING
Application: WB
Impact Factor: 3.70
Species: Mouse,Human
PMID: 40651685
The combination of gemcitabine and albumin-bound paclitaxel effectively inhibits de novo lipogenesis in pancreatic cancer cells by targeting the AMPK/SREBP1 pathway
Published: 2024-12-17
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 5.30
Species: Human
PMID: 39694195
Transcriptomics and Molecular Docking Reveal the Potential Mechanism of Lycorine Against Pancreatic Cancer
Published: 2023-10-04
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 7.90
Species: Human
PMID: 37839227
Comparison of protective effects of hesperetin and pectolinarigenin on high-fat diet-induced hyperlipidemia and hepatic steatosis in Golden Syrian hamsters
Published: 2022-10-31
Journal: EXPERIMENTAL ANIMALS
Application: WB
Impact Factor: 1.80
Species: Hamster
PMID: 36310057
Fat mass and obesity-associated protein regulates lipogenesis via m6A modification in fatty acid synthase mRNA
Published: 2020-10-20
Journal: CELL BIOLOGY INTERNATIONAL
Application: WB
Impact Factor: 2.57
Species: Human
PMID: 33079435
Increased HAGLR expression promotes non–small cell lung cancer proliferation and invasion via enhanced de novo lipogenesis
Published: 2017-04-26
Journal: TUMOR BIOLOGY
Application: WB
Impact Factor: 3.65
Species: Human
PMID: 28443464
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Product Promise
We promise Anti-Fatty Acid Synthase/FASN Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9865
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026