Product Info Summary
SKU: | A00188-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Flt3/Cd135 Antibody
View all Flt-3/Flk-2/CD135 Antibodies
SKU/Catalog Number
A00188-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Flt3/Cd135 Antibody catalog # A00188-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Flt3/Cd135 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00188-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human FLT3. AA range:565-614
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00188-1 is reactive to FLT3 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
112903 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00188-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HEPG2 cells using Flt3 Polyclonal Antibody diluted at 1:1000
Click image to see more details
Western blot analysis of the lysates from K562 cells using FLT3 antibody.
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Western blot analysis of lysates from LOVO cells, using FLT3 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For FLT3 (Source: Uniprot.org, NCBI)
Gene Name
FLT3
Full Name
Receptor-type tyrosine-protein kinase FLT3
Weight
112903 MW
Superfamily
protein kinase superfamily
Alternative Names
Receptor-type tyrosine-protein kinase FLT3;2.7.10.1;FL cytokine receptor;Fetal liver kinase-2;FLK-2;Fms-like tyrosine kinase 3;FLT-3;Stem cell tyrosine kinase 1;STK-1;CD135;FLT3;CD135, FLK2, STK1; FLT3 CD135, FLK-2, FLK2, STK1 fms related receptor tyrosine kinase 3 receptor-type tyrosine-protein kinase FLT3|CD135 |FL cytokine receptor|fetal liver kinase 2|fms related tyrosine kinase 3|fms-like tyrosine kinase 3|growth factor receptor tyrosine kinase type III|stem cell tyrosine kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FLT3, check out the FLT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FLT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Flt3/Cd135 Antibody (A00188-1)
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No publications found for A00188-1
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Customer Q&As
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15 Customer Q&As for Anti-Flt3/Cd135 Antibody
Question
I am interested in to test anti-Flt3/Cd135 antibody A00188-1 on human lymphocyte for research purposes, then I may be interested in using anti-Flt3/Cd135 antibody A00188-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The products we sell, including anti-Flt3/Cd135 antibody A00188-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-22
Question
Is there a BSA free version of anti-Flt3/Cd135 antibody A00188-1 available?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Flt3/Cd135 antibody A00188-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-01
Question
I was wanting to use your anti-Flt3/Cd135 antibody for WB for human lymphocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphocyte identification?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
As indicated on the product datasheet, A00188-1 anti-Flt3/Cd135 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human lymphocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-23
Question
My question regarding product A00188-1, anti-Flt3/Cd135 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00188-1 anti-Flt3/Cd135 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-17
Question
My colleagues were well pleased with the WB result of your anti-Flt3/Cd135 antibody. However we have observed positive staining in bone marrow membrane using this antibody. Is that expected? Could you tell me where is FLT3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
From literature, bone marrow does express FLT3. Generally FLT3 expresses in membrane. Regarding which tissues have FLT3 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7507245
Lymphocyte, Pubmed ID: 8394751, 15057823
Testis, Pubmed ID: 2004790
Boster Scientific Support
Answered: 2019-07-04
Question
I see that the anti-Flt3/Cd135 antibody A00188-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-17
Question
Does anti-Flt3/Cd135 antibody A00188-1 work for WB with lymphocyte?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
According to the expression profile of lymphocyte, FLT3 is highly expressed in lymphocyte. So, it is likely that anti-Flt3/Cd135 antibody A00188-1 will work for WB with lymphocyte.
Boster Scientific Support
Answered: 2019-03-12
Question
Is a blocking peptide available for product anti-Flt3/Cd135 antibody (A00188-1)?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
We do provide the blocking peptide for product anti-Flt3/Cd135 antibody (A00188-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-17
Question
Is this A00188-1 anti-Flt3/Cd135 antibody reactive to the isotypes of FLT3?
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
The immunogen of A00188-1 anti-Flt3/Cd135 antibody is Synthesized peptide derived from human Flt3 around the non-phosphorylation site of Y599. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-09
Question
We are currently using anti-Flt3/Cd135 antibody A00188-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
The anti-Flt3/Cd135 antibody (A00188-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-27
Question
Here is the WB image, lot number and protocol we used for lymphocyte using anti-Flt3/Cd135 antibody A00188-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-18
Question
Will A00188-1 anti-Flt3/Cd135 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
You can see on the product datasheet, A00188-1 anti-Flt3/Cd135 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-28
Question
We are interested in using your anti-Flt3/Cd135 antibody for hemopoiesis studies. Has this antibody been tested with western blotting on hepg2 cells? We would like to see some validation images before ordering.
G. Yang
Verified customer
Asked: 2017-03-13
Answer
Thank you for your inquiry. This A00188-1 anti-Flt3/Cd135 antibody is tested on hepg2 cells. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-03-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphocyte using anti-Flt3/Cd135 antibody A00188-1. Let me know if you need anything else.
B. Patel
Verified customer
Asked: 2017-01-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-04
Question
We have observed staining in human cerebellar hemisphere. Are there any suggestions? Is anti-Flt3/Cd135 antibody supposed to stain cerebellar hemisphere positively?
F. Krishna
Verified customer
Asked: 2015-08-25
Answer
According to literature cerebellar hemisphere does express FLT3. According to Uniprot.org, FLT3 is expressed in cerebellar hemisphere, bone marrow, lymphocyte, testis, among other tissues. Regarding which tissues have FLT3 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7507245
Lymphocyte, Pubmed ID: 8394751, 15057823
Testis, Pubmed ID: 2004790
Boster Scientific Support
Answered: 2015-08-25