Anti-Forkhead box protein P3 FOXP3 Antibody

Immunohistochemistry findings from tissue samples of CRC patients before and after MTE treatment. (A) Shown are the findings from one patient. Results of other 16 patients are shown in Figure S1 . (B) The bar graph compares the expression of PD-1, PD-L1, TGF-β1(tumor), and FOXP3 before and after MTE treatment in all 17 patients. (C) Serum levels of IL-2, IL-10 and TGF-β1 were compared before and after treatment in all 17 patients. Cytokine concentrations were normalized against the untreated group. * P < 0.05.
Index in PubMed under a CC BY license. PMID: 37649480

Western blotting and ELISA of HCT116, LoVo and Jurkat T cells treated with MTE treated in single culture or co-culture condition. (A) Western blot analysis of PD-L1, TGF-β1 expression in HCT116 or LoVo cells, single cultured or co-cultured with Jurkat T cells and treated with MTE, which were labeled in top row of blots or bottom row of column chart. (B) Western blot analysis of PD-1 and FOXP3 expression in Jurkat T cells single cultured or co-cultured with HCT116 or LoVo cells, and treated with MTE, which were labeled in top row of blots or bottom row of column chart. (C) ELISA analysis of supernatants from single cultures or co-cultures. In single culture, supernatants of HCT116 or LoVo cells was used to test TGF-β1 secretion, and supernatants of Jurkat T cells was used to test IL-2 and IL-10 secretion levels.* P < 0.05, ** P < 0.01.
Index in PubMed under a CC BY license. PMID: 37649480

IHC analysis of FOXP3 using anti-FOXP3 antibody (RP1032).
FOXP3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXP3 Antibody (RP1032) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of FOXP3 using anti-FOXP3 antibody (RP1032).
FOXP3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXP3 Antibody (RP1032) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.