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- Table of Contents
FOXP3 antibody (RP1032) performs well in IHC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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IHC analysis of FOXP3 using anti-FOXP3 antibody (RP1032).
FOXP3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXP3 Antibody (RP1032) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
FOXP3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXP3 Antibody (RP1032) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Forkhead box protein P3 FOXP3 Antibody
FOXP3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC |
Tested Applications
FOXP3 antibody was positively detected by IHC in:
human tonsil tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Human
Tested
Recommend
Monkey
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-Forkhead box protein P3 FOXP3 Antibody
View all FOXP3 Antibodies
View all FOXP3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FOXP3 recombinant protein (Position: H101-P431). Human FOXP3 shares 88% amino acid (aa) sequence identity with mouse FOXP3.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
47.2 kDa
Background of FOXP3
FOXP3 (forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome (specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.
Cite This Product
Anti-Forkhead box protein P3 FOXP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1032)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Cow
92%
High Homology
Sheep
91%
High Homology
Dog
91%
High Homology
Rabbit
89%
Moderate Homology
Mouse
87%
Moderate Homology
Rat
85%
Moderate Homology
Zebrafish
41%
Low homology
Drosophila
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for FOXP3 antibody
Publications
Application:
Species:
FUT7 improves intestinal immune homeostasis in IBD by enhancing Treg intestinal homing and immunosuppression
Published: 2025-04-01
Journal: GASTROENTEROLOGY
Application: IHC
Impact Factor: 25.10
Species: Mouse
PMID: 40180293
Phillygenin regulates the colorectal cancer tumor microenvironment by inhibiting hypoxia-inducible factor 1 alpha
Published: 2024-12-10
Journal: CYTOTECHNOLOGY
Application: WB,IHC
Impact Factor: 2.00
Species: Human,Mouse
PMID: 39669690
Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancer
Published: 2023-08-15
Journal: Frontiers in Immunology
Application: IHC
Impact Factor: 7.30
Species: Human
PMID: 37649480
Regulatory T cells promote functional recovery after spinal cord injury by alleviating microglia inflammation via STAT3 inhibition
Published: 2023-03-13
Journal: CNS Neuroscience & Therapeutics
Application: IF
Impact Factor: 5.50
Species: Mouse
PMID: 36914969
Manipulating adrenergic stress receptor signaling to enhance immunosuppression and prolong survival of vascularized composite tissue transplants
Published: 2022-08-22
Journal: Clinical and Translational Medicine
Application: IHC
Impact Factor: 8.55
Species: Mouse
PMID: 35994413
Prognosis significance of indoleamine 2, 3-dioxygenase, programmed death ligand-1 and tumor-infiltrating immune cells in microenvironment of breast cancer
Published: 2020-04-21
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IHC
Impact Factor: 3.94
Species: Human
PMID: 32330866
Effect of compound sophorae decoction on dextran sodium sulfate (DSS)-induced colitis in mice by regulating Th17/Treg cell balance
Published: 2018-11-30
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: IHC
Impact Factor: 3.46
Species: Mouse
PMID: 30551499
Inhibition of microRNA-155 attenuates concanavalin-A-induced autoimmune hepatitis by regulating Treg/Th17 cell differentiation
Published: 2018-10-05
Journal: CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
Application: WB
Impact Factor: 2.21
Species: Mouse
PMID: 30290123
Fibroblast activation proteins-α suppress tumor immunity by regulating T cells and tumor-associated macrophages
Published: 2017-12-19
Journal: EXPERIMENTAL AND MOLECULAR PATHOLOGY
Application: IHC
Impact Factor: 2.42
Species: Human
PMID: 29273462
IL-35 improves Treg-mediated immune suppression in atherosclerotic mice
Published: 2016-09-01
Journal: Experimental and Therapeutic Medicine
Application: IHC,FC
Impact Factor: 1.28
Species: Mouse
PMID: 27698748
T2 enhances in situ level of Foxp3+ regulatory cells and modulates inflammatory cytokines in Crohn's disease
Published: 2013-12-22
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IHC
Impact Factor: 2.42
Species: Human
PMID: 24369313
Potential role of plasmacytoid dendritic cells for FOXP3+ regulatory T cell development in human colorectal cancer and tumor draining lymph node
Published: 2013-09-06
Journal: PATHOLOGY RESEARCH AND PRACTICE
Application: IHC
Impact Factor: 1.21
Species: Human
PMID: 24080284
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Product Promise
We promise Anti-Forkhead box protein P3 FOXP3 Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
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RP1032
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026