Product Info Summary
| SKU: | PA1577 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Forkhead box protein P3 FOXP3 Antibody Picoband®
SKU/Catalog Number
PA1577
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Forkhead box protein P3 FOXP3 Antibody catalog # PA1577. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Forkhead box protein P3 FOXP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1577)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human FOXP3, different from the related mouse sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1577 is reactive to FOXP3 in Human
Observed Molecular Weight
55 kDa
Calculated molecular weight
47.2 kDa
Background of FOXP3
FOXP3 (forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome (specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1577 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Cell, SGC Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-FOXP3 antibody, PA1577, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SGC Cell Lysate
Specific Publications For Anti-Forkhead box protein P3 FOXP3 Antibody Picoband® (PA1577)
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2 Customer Q&As for Anti-Forkhead box protein P3 FOXP3 Antibody Picoband®
Question
I am interested in using your anti-FOXP3 antibody for t cell homeostasis studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
We appreciate your inquiry. This PA1577 anti-FOXP3 antibody is tested on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-07
Question
We are currently using anti-FOXP3 antibody PA1577 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The anti-FOXP3 antibody (PA1577) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03


