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- Table of Contents
FOXP3 antibody (PA1577) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Anti-FOXP3 antibody, PA1577, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SGC Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: SGC Cell Lysate
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Anti-Forkhead box protein P3 FOXP3 Antibody Picoband®
FOXP3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
FOXP3 antibody was positively detected by WB in:
HELA Cell, SGC Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Forkhead box protein P3 FOXP3 Antibody Picoband®
View all FOXP3 Antibodies
View all FOXP3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human FOXP3, different from the related mouse sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
47.2 kDa
Background of FOXP3
FOXP3 (forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome (specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.
Cite This Product
Anti-Forkhead box protein P3 FOXP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1577)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
94%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Dog
94%
High Homology
Mouse
88%
Moderate Homology
Rat
88%
Moderate Homology
Zebrafish
41%
Low homology
Drosophila
29%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FOXP3 antibody
Publications
Application:
IL-35 improves Treg-mediated immune suppression in atherosclerotic mice
PMID: 27698748
Decreased FOXP3+ and GARP+ Tregs to neoadjuvant chemotherapy associated with favorable prognosis in advanced gastric cancer
PMID: 27366089
LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn?s disease
PMID: 24289115
Wang B, Dai S, Dong Z, Sun Y, Song X, Guo C, Zhu F, Wang Q, Zhang L. Plos One. 2014 Jan 30;9(1):E87787. Doi: 10.1371/Journal.Pone.0087787. Ecollection 2014. The Modulation Of Endoplasmic Reticulum Stress By Chemical Chaperone Upregulates Immune Ne...
PMID: 24498194
Hu M, Li K, Maskey N, Xu Z, Peng C, Wang B, Li Y, Yang G. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4685-94. Ecollection 2014. Decreased Intratumoral Foxp3 Tregs And Increased Dendritic Cell Density By Neoadjuvant Chemotherapy Associated With Favora...
PMID: 25197340
Gai Xd, Song Y, Li C, Lei Ym, Yang B. Pathol Res Pract. 2013 Dec;209(12):774-8. Doi: 10.1016/J.Prp.2013.08.011. Epub 2013 Sep 6. Potential Role Of Plasmacytoid Dendritic Cells For Foxp3+ Regulatory T Cell Development In Human Colorectal Cancer And...
PMID: 24080284
Gai Xd, Li C, Song Y, Lei Ym, Yang Bx. Biomed Rep. 2013 Mar;1(2):207-212. Epub 2012 Oct 29. In Situ Analysis Of Foxp3+ Regulatory T Cells And Myeloid Dendritic Cells In Human Colorectal Cancer Tissue And Tumor-Draining Lymph Node.
PMID: 24648920
Fu Hy, Li C, Yang W, Gai Xd, Jia T, Lei Ym, Li Y. Acta Histochem. 2013 Mar;115(2):151-7. Doi: 10.1016/J.Acthis.2012.06.002. Epub 2012 Jun 29. Foxp3 And Tlr4 Protein Expression Are Correlated In Non-Small Cell Lung Cancer: Implications For Tumor Pr...
PMID: 22749378
Li G, Ren J, Wang G, Gu G, Hu D, Ren H, Hong Z, Wu X, Liu S, Li J. Int Immunopharmacol. 2014 Feb;18(2):244-8. Doi: 10.1016/J.Intimp.2013.12.014. Epub 2013 Dec 22. T2 Enhances In Situ Level Of Foxp3+ Regulatory Cells And Modulates Inflammatory Cyto...
PMID: 24369313
Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...
PMID: 26064167
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Product Promise
We promise Anti-Forkhead box protein P3 FOXP3 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1577
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 26, 2026