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Product Info Summary
| SKU: | A05330 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Frequenin Ncs1 Antibody
SKU/Catalog Number
A05330
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Frequenin Ncs1 Antibody (Catalog # A05330). Tested in IF applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Frequenin Ncs1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05330)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Frequenin (recombinant from Mouse with extensive post-translational modifications)
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05330 is reactive to NCS1 in Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
21.9 kDa
Background of NCS1
Frequenin, or Neuronal calcium sensor, is a regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. It directly regulates GRK1 (RHOK), but not GRK2 to GRK5. It can substitute for calmodulin and it stimulates PI4KB kinase activity. It is involved in long-term synaptic plasticity through its interaction with PICK1. Ncs1 may also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05330 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IF Microscopy: 1:100-1:2500
WB: 1:500- 1:2,000
This product was assayed by immunoblot and found to be reactive against Frequenin at a dilution of 1:5000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-Frequenin is suitable for the detection by immunoblot of human, mouse and rat Frequenin. Anti-Frequenin has also been tested for use in IF using Hippocampal neurons of 17 day old NMRI mice.
Validation Images & Assay Conditions
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Hippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously (G. Grosse et al., (2000) J. Neurosci. 20: 1869-1882). The figure shows the distribution of neuronal calcium sensor-1 (NCS-1) (red) and synaptobrevin (green) in hippocampal cell cultures after 19 d in vitro. Numerous synapses immunoreactive for synaptobrevin also show NCS-1 immunoreactivity. NCS-1 derived from invertebrate homologs has been referred to as frequenin.
Specific Publications For Anti-Frequenin Ncs1 Antibody (A05330)
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7 Customer Q&As for Anti-Frequenin Ncs1 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for dorsolateral prefrontal cortex using anti-Frequenin antibody A05330. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-16
Question
you antibody to test anti-Frequenin antibody A05330 on rat dorsolateral prefrontal cortex for research purposes, then I may be interested in using anti-Frequenin antibody A05330 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The products we sell, including anti-Frequenin antibody A05330, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-12
Question
We are currently using anti-Frequenin antibody A05330 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
The anti-Frequenin antibody (A05330) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-29
Question
Would A05330 anti-Frequenin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
You can see on the product datasheet, A05330 anti-Frequenin antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-20
Question
See attached the WB image, lot number and protocol we used for dorsolateral prefrontal cortex using anti-Frequenin antibody A05330. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-05
Question
Does anti-Frequenin antibody A05330 work on canine IF with dorsolateral prefrontal cortex?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
Our lab technicians have not validated anti-Frequenin antibody A05330 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Frequenin antibody A05330 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine dorsolateral prefrontal cortex in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-03
Question
I was wanting to use your anti-Frequenin antibody for WB for rat dorsolateral prefrontal cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat dorsolateral prefrontal cortex identification?
A. Banerjee
Verified customer
Asked: 2016-04-13
Answer
It shows on the product datasheet, A05330 anti-Frequenin antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat dorsolateral prefrontal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-13
