Anti-Frequenin Ncs1 Antibody

Product Name

Anti-Frequenin Ncs1 Antibody

View all Frequenin Antibodies

SKU/Catalog Number

A05330

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Frequenin Ncs1 Antibody (Catalog # A05330). Tested in IF applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Frequenin Ncs1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05330)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Frequenin (recombinant from Mouse with extensive post-translational modifications)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05330 is reactive to Ncs1 in Mouse

Applications

A05330 is guaranteed for IF Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

21.879kDa

Background of Frequenin

Frequenin, or Neuronal calcium sensor, is a regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. It directly regulates GRK1 (RHOK), but not GRK2 to GRK5. It can substitute for calmodulin and it stimulates PI4KB kinase activity. It is involved in long-term synaptic plasticity through its interaction with PICK1. Ncs1 may also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IF Microscopy: 1:100-1:2500
WB: 1:500- 1:2,000

Validation Images & Assay Conditions

Click image to see more details

Hippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously (G. Grosse et al., (2000) J. Neurosci. 20: 1869-1882).

Gene/Protein Information For Ncs1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp761L1223; FREQFLUP; frequenin (Drosophila) homolog; frequenin homolog (Drosophila); Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein; NCS-1; neuronal calcium sensor 1 Ncs1|9430075O15Rik, A730032G13Rik, AI836659, Fre, Freq, Mfreq, NCS, NCS-1|neuronal calcium sensor 1|neuronal calcium sensor 1|frequenin homolog

For more info on Ncs1, check out the Ncs1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ncs1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Ncs1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis