SKU MA1044
Size 100μg/vial
Reactivity Human, Rabbit
Clonality Monoclonal
Clone Number GS-2C4
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Gelsolin Antibody (Monoclonal, GS-2C4)
SKU/Catalog Number MA1044
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Gelsolin, gelsolin (GSN) detection. Tested with WB, IHC-P, IHC-F in Human; rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Gelsolin Antibody (Monoclonal, GS-2C4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1044)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Human plasma gelsolin.
Reactivity Human, Rabbit

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, rabbit, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, rabbit, -
Western blot, 0.5-1μg/ml, Human, rabbit

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1044 1 IHC anti gelsolin antibody monoclonal gs 2c4.jpg

Anti- Gelsolin antibody, MA1044, IHC(P)
IHC(P): Human Lung Cancer Tissue

/antibody/ma1044 2 IHC anti gelsolin antibody monoclonal gs 2c4.jpg

Anti- Gelsolin antibody, MA1044, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

/m/a/ma1044 3.jpg

Figure 3. Western blot analysis of Gelsolin using anti-Gelsolin antibody (MA1044).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human A549 whole cell lysate,
Lane 3: monkey COS-7 whole cell lysate,
Lane 4: human Caco-2 whole cell lysate,
Lane 5: human HepG2 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Gelsolin antigen affinity purified monoclonal antibody (Catalog # MA1044) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 90KD. The expected band size for Gelsolin is at 86KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q68FP1
Gene Name GSN
Protein Name Gelsolin
Tissue Specificity GSN: Phagocytic cells, platelets, fibroblasts,nonmuscle cells, smooth and skeletal muscle cells.
Alternative Names Gelsolin;Actin-depolymerizing factor;ADF;Brevin;Gsn;
Subcellular Localization Isoform 1: Secreted .
Molecular Weight 86068 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis (By similarity). .
Research Areas Actin Binding Proteins, Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction

*You can search these to find other products in these research areas.
Background Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.

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Order Product (MA1044)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

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Troubleshooting

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Publications

Proteomics of acute heart failure in a rat post-myocardial infarction model

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.