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Product Info Summary
| SKU: | MA1044 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rabbit |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4)
SKU/Catalog Number
MA1044
BM1620 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4) catalog # MA1044. Tested in IHC, WB applications. This antibody reacts with Human, Rabbit.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1044)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
GS-2C4
Isotype
Mouse IgG1
Immunogen
Human plasma gelsolin.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1044 is reactive to Gsn in Human, Rabbit
Observed Molecular Weight
90 kDa
Calculated molecular weight
86.1 kDa
Background of Gsn
Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1044 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, rabbit
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, rabbit, -
Western blot, 0.5-1μg/ml, Human, rabbit
Positive Control
WB: human Hela whole cell, human A549 whole cell, monkey COS-7 whole cell, human Caco-2 whole cell, human HepG2 whole cell
IHC: Human Lung Cancer Tissue, Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
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Anti-Gelsolin antibody, MA1044, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-Gelsolin antibody, MA1044, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Western blot analysis of Gelsolin using anti-Gelsolin antibody (MA1044).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human A549 whole cell lysate,
Lane 3: monkey COS-7 whole cell lysate,
Lane 4: human Caco-2 whole cell lysate,
Lane 5: human HepG2 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Gelsolin antigen affinity purified monoclonal antibody (Catalog # MA1044) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 90KD. The expected band size for Gelsolin is at 86KD.
Specific Publications For Anti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4) (MA1044)
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