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- Table of Contents
GCG antibody (PB9705) performs well in IF, IHC and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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IHC analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB9705).
Glucagon/GCG was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Glucagon/GCG Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Glucagon/GCG was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Glucagon/GCG Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-Glucagon/GCG Antibody
GCG antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC |
Tested Applications
GCG antibody was positively detected by IHC in:
mouse pancreas tissue, rat pancreas tissue, human pancreatic cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GCG antibody was positively detected by IF in:
mouse pancreas tissue, rat pancreas tissue
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunofluorescence
2μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Mouse
Tested
Tested
Rat
Tested
Tested
Human
Tested
Recommend
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Dog
Predicted
Predicted
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-Glucagon/GCG Antibody
View all GCG Antibodies
View all GCG Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Glucagon/GCG, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
20.9 kDa
Background of GCG
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
Cite This Product
Anti-Glucagon/GCG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9705)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
79%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for GCG antibody
Immunofluorescence (IF) protocol for GCG antibody
Publications
Application:
Species:
Irisin alleviates the pyroptosis of β cells in T2DM by inhibiting NLRP3 inflammasome through regulating miR-19b-3p/SOCS3/STAT3 axis mediated autophagy
Published: 2024-08-15
Journal: IUBMB LIFE
Application: IF
Impact Factor: 3.70
Species: Mouse
PMID: 39143849
Mannogalactoglucan from mushrooms protects pancreatic islets via restoring UPR and promotes insulin secretion in T1DM mice
Published: 2024-02-08
Journal: Food Science and Human Wellness
Application: IF
Impact Factor: 5.60
Species: Mouse
Glutathione prevents chronic oscillating glucose intake-induced β-cell dedifferentiation and failure
Published: 2019-04-11
Journal: Cell Death & Disease
Application: IHC
Impact Factor: 5.96
Species: Rat
PMID: 30975975
Generation and Biological Characteristics of a Mouse Model of Breast Cancer that Copresents with Diabetes Mellitus
Published: 2018-10-09
Journal: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
Application: IHC
Impact Factor: 1.37
Species: Mouse
PMID: 30299597
Effect of acute exposure to PFOA on mouse liver cells in vivo and in vitro
Published: 2017-09-08
Journal: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Application: IHC
Impact Factor: 2.74
Species: Mouse
PMID: 28887612
The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlying signaling mechanisms in a rat model of diabetes mellitus
Published: 2016-10-10
Journal: Scientific Reports
Application: IF
Impact Factor: 5.23
Species: Rat
PMID: 27721485
A refined-JinQi-JiangTang tablet ameliorates prediabetes by reducing insulin resistance and improving beta cell function in mice
Published: 2013-11-25
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC
Impact Factor: 2.76
Species: Mouse
PMID: 24286962
Effect of catch-up growth after food restriction on the entero-insular axis in rats
Published: 2010-05-26
Journal: Nutrition & Metabolism
Application: IHC
Impact Factor: 2.62
Species: Rat
PMID: 20504302
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Product Promise
We promise Anti-Glucagon/GCG Antibody performs excellently in relevant applications for IF, IHC. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PB9705
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026