Anti-Glucagon/GCG Antibody

IHC analysis of GLP1 using anti-GLP1 antibody (PB9705).
GLP1 was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Expression of GLP-1 and GIP in ileum. ( A ) Images (×400) of immunofluorescence staining of GLP-1 (glucagon-like peptide-1) and GIP (gastric inhibitory polypeptide) expression in ileum in the Control, DM and DMC groups. ( B ) Immunofluorescence results (×400) indicating GIP expression. (scale bar: 20μm). ( C ) Quantification of the GLP-1 fluorescence intensity (integrated density per stained area). ( D ) Quantification of the GIP fluorescence intensity (integrated density per stained area). *p < 0.05 vs Control, # p < 0.05 vs DM; mean ± SEM.
Index in PubMed under a CC BY license. PMID: 27721485

GLP-1 and GIP concentrations in the serum and mRNA levels of GCG and GIP. ( A ) GLP-1 concentrations in the serum in the in the Control, DM and DMC groups. ( B ) GCG (the gene encoding GLP-1) mRNA levels in ileum. ( C ) GIP concentrations in the serum in the five groups. ( D ) GIP mRNA levels in ileum. ( E ) Insulin levels in the serum in the five groups. *p < 0.05, **p < 0.01 vs Control, # p < 0.05, ## p < 0.01 vs DM; mean ± SEM.
Index in PubMed under a CC BY license. PMID: 27721485

HE Staining (×200 magnification) and glucagon staining (×400 magnification) in rat ileum . Ileal villus observed in the CUGFR0 (A1) and CUGFR2 (A2) groups with HE staining and NC4 (B1) and CUGFR4 (B2) groups with glucagon staining. Arrow shows a glucagon-positive cell.
Index in PubMed under a CC BY license. PMID: 20504302

Alterations of the protein levels of the components of the GSK-3β—β-catenin—TCF7L2—GLP-1 axis under varying glucose conditions. ( A ) Molecular structure of Emodin. ( B ) Relative protein levels of p-GSK-3β, GSK-3β, β-catenin and TCF7L2 in Control and Emodin groups under different glucose conditions, detected by the western blot analysis. ( C ) Relative mRNA expression of β-catenin and TCF7L2 in Control and Emodin groups under different glucose conditions, detected by real-time PCR. ( D ) GLP-1 and GIP concentration measured by ELISA in the cell supernatant in Control and Emodin groups under different glucose conditions. *p < 0.05, **p < 0.01 vs control under no glucose, # p < 0.05, ## p < 0.01 vs control under low glucose, &p < 0.05, && p < 0.01 vs control under high glucose; mean ± SEM. ( E,F ) Inhibition of TCF7L2 and GSK-3β abolished DMC’s effects. ( E ) Effects of emodin (the main component of DMC) and SB216763 (a specific GSK-3β inhibitor) on the protein levels of β-catenin and TCF7L2 under HG (high glucose) conditions. ( F ) Relative mRNA expression of β-catenin and TCF7L2 in the three groups. ( G ) Relative protein expression of TCF7L2 in HG, HG + emodin and HG + emodin + TCF7L2 SiRNA groups. ( H ) GLP-1 and GIP concentration measured by ELISA in the cell supernatant in the three groups. *p < 0.05, **p < 0.01 vs HG, #p < 0.05, ##p < 0.01 vs HG + Emodin; mean ± SEM.
Index in PubMed under a CC BY license. PMID: 27721485

Effect of catch-up growth on glucagon-positive cells per high-power field . To quantify the expression of glucagon, an average of 50 sections of rat ileum from each of two animals were examined. The number of cells immunoreactive for glucagon was determined. Data are expressed as means ± SD. *P ≤ 0.05 versus NC group; **P ≤ 0.01 versus NC group.
Index in PubMed under a CC BY license. PMID: 20504302

IHC analysis of GLP1 using anti-GLP1 antibody (PB9705).
GLP1 was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IHC analysis of GLP1 using anti-GLP1 antibody (PB9705).
GLP1 was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IF analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB9705) and anti-Insulin antibody (MA1052).
Glucagon/GCG was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Glucagon/GCG Antibody (PB9705) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB9705) and anti-Insulin antibody (MA1052).
Glucagon/GCG was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Glucagon/GCG Antibody (PB9705) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.