Product Info Summary
SKU: | PB9705 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC |
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Product info
Product Name
Anti-GLP1/GCG Antibody Picoband™
SKU/Catalog Number
PB9705
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GLP1/GCG Antibody Picoband™ catalog # PB9705. Tested in IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GLP1/GCG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9705)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GLP1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9705 is reactive to GCG in Human, Mouse, Rat
Applications
PB9705 is guaranteed for IF, IHC Boster Guarantee
Observed Molecular Weight
10 kDa
Calculated molecular weight
20.909kDa
Background of GCG
GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue. It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunofluorescence, 2μg/ml, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of GLP1 using anti-GLP1 antibody (PB9705).
GLP1 was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of GLP1 using anti-GLP1 antibody (PB9705).
GLP1 was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of GLP1 using anti-GLP1 antibody (PB9705).
GLP1 was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of GLP1 using anti-GLP1 antibody (PB9705)
GLP1 was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. IF analysis of GLP1 using anti-GLP1 antibody (PB9705)
GLP1 was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For GCG (Source: Uniprot.org, NCBI)
Gene Name
GCG
Full Name
Glucagon
Weight
20.909kDa
Superfamily
glucagon family
Alternative Names
GCG; glicentin-related polypeptide; GLP1; GLP-1; Glucagon; glucagon-like peptide 1; glucagon-like peptide 2; GRPP GCG GLP-1, GLP1, GLP2, GRPP glucagon pro-glucagon|glicentin-related polypeptide|glucagon-like peptide 1|glucagon-like peptide 2|preproglucagon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GCG, check out the GCG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GCG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GLP1/GCG Antibody Picoband™ (PB9705)
Hello CJ!
PB9705 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huo K,Li X,Hu W,Song X,Zhang D,Zhang X,Chen X,Yuan J,Zuo J,Wang X.RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis.Front Endocrinol (Lausanne). 2020 Apr 9;11:194.doi:10.3389/fendo.2020.00194.PMID
Species: Rat
PB9705 usage in article: APP:IHC, SAMPLE:PANCREATIC TISSUE, DILUTION:1:100
The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlying signaling mechanisms in a rat model of diabetes mellitus
Generation and Phenotype Identification of PAX4 Gene Knockout Rabbit by CRISPR/Cas9 System
Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil?lymphocyte ratio in rats with or without hidden diabetes mellitus
Low dose doxycycline decreases systemic inflammation and improves glycemic control, lipid profiles, and islet morphology and function in db/db mice
Chen Ll, Yang Wh, Zheng J, Hu X, Kong W, Zhang Hh. Nutr Metab (Lond). 2010 May 26;7:45. Doi: 10.1186/1743-7075-7-45. Effect Of Catch-Up Growth After Food Restriction On The Entero-Insular Axis In Rats.
Lan F, Yue X, Han L, Shi Z, Yang Y, Pu P, Yao Z, Kang C. Int J Oncol. 2012 Feb;40(2):519-26. Doi: 10.3892/Ijo.2011.1215. Epub 2011 Sep 28. Genome-Wide Identification Of Tcf7L2/Tcf4 Target Mirnas Reveals A Role For Mir-21 In Wnt-Driven Epithelial C...
Zhang Y, Ye C, Wang G, Gao Y, Tan K, Zhuo Z, Liu Z, Xia H, Yang D, Li P. Biomed Res Int. 2013;2013:526367. Doi: 10.1155/2013/526367. Epub 2013 May 27. Kidney-Targeted Transplantation Of Mesenchymal Stem Cells By Ultrasound-Targeted Microbubble Des...
Tang Mm, Zhu Qe, Fan Wz, Zhang Sl, Li Dz, Liu Lz, Chen M, Zhang M, Zhou J, Wei Cj. Mol Ther. 2011 Jan;19(1):60-6. Doi: 10.1038/Mt.2010.187. Epub 2010 Sep 14. Intra-Arterial Targeted Islet-Specific Expression Of Sirt1 Protects ?? Cells From Strepto...
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-GLP1/GCG Antibody Picoband™
Question
We are currently using anti-GLP1/GCG antibody PB9705 for mouse tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
The anti-GLP1/GCG antibody (PB9705) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-06