|Validated Species:||Human, Mouse, Rabbit, Rat|
Data & Images
|Product Name||Anti-Glucagon Antibody (Monoclonal, K79bB10)|
|Description||Mouse IgG monoclonal antibody for Glucagon, glucagon (GCG) detection. Tested with IHC-P in Human, mouse, rat, rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Glucagon Antibody (Monoclonal, K79bB10) K79bB10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1047)|
|Replacement Item||This antibody may replace the following items: sc-130624|sc-13091|sc-167994|sc-167996|sc-167998|sc-26637|sc-514592|sc-57164|sc-57165|sc-57166|sc-57167|sc-57168|sc-57169|sc-57170|sc-57171|sc-57510|sc-65388|sc-65389|sc-71150|sc-71151|sc-71152|sc-73508|sc-7779|sc-7779-R|sc-7780|sc-7781|sc-7782|sc-80570|sc-80574|sc-80602|sc-80603|sc-80604 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rabbit, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Polymerized porcine glucagon.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||20846 MW|
|Protein Function||Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. .|
|Tissue Specificity||Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. .|
|Sequence Similarities||Belongs to the glucagon family.|
|Alternative Names||Glucagon;Glicentin;Glicentin-related polypeptide;GRPP;Oxyntomodulin;OXM;OXY;Glucagon;Glucagon-like peptide 1;GLP-1;Glucagon-like peptide 1(7-37);GLP-1(7-37);Glucagon-like peptide 1(7-36);GLP-1(7-36);Glucagon-like peptide 2;GLP-2;Gcg;|
|Research Areas|||signal transduction|growth factors/hormones| stem cells|lineage markers|endoderm| cancer|tumor biomarkers| developmental biology|lineage specification|cancer metabolism|metabolic signaling pathway|integration of energy metabolism| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways|integration of energy|types of disease||
Background for Glucagon
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Glucagon Antibody (Monoclonal, K79bB10) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, rabbit, By Heat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Glucagon Antibody (Monoclonal, K79bB10) Images
Click the images to enlarge.
IHC(P): Rat Pancreas Tissue