Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10)

Boster Bio Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10) catalog # MA1047. Tested in IHC applications. This antibody reacts with Human, Mouse, Rabbit, Rat. Cited in 12 publication(s).

Product Info Summary

SKU: MA1047
Size: 100μg/vial
Reactive Species: Human, Mouse, Rabbit, Rat
Host: Mouse
Application: IHC

Product Name

Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10)

See all Gcg products

SKU/Catalog Number

MA1047

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10) catalog # MA1047. Tested in IHC applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1047)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

K79bB10

Isotype

Mouse IgG1

Immunogen

Polymerized porcine glucagon.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1047 is reactive to Gcg in Human, Mouse, Rabbit, Rat

Applications

MA1047 is guaranteed for IHC Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, rabbit, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For Gcg (Source: Uniprot.Org, NCBI)

Uniprot ID

P06883

Gene ID

24952

Gene Name

Gcg

Full Name

Glucagon

Weight

20.846kDa

Superfamily

glucagon family

Alternative Names

GCG; glicentin-related polypeptide; GLP1; GLP-1; Glucagon; glucagon-like peptide 1; glucagon-like peptide 2; GRPP Gcg|GLP-1, Glp1|glucagon|pro-glucagon|glucagon-like peptide-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Gcg, check out the Gcg Infographic

Gcg infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Gcg: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1047 has been cited in 12 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Huo K,Li X,Hu W,Song X,Zhang D,Zhang X,Chen X,Yuan J,Zuo J,Wang X.RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis.Front Endocrinol (Lausanne). 2020 Apr 9;11:194.doi:10.3389/fendo.2020.00194.PMID
Species: Rat
MA1047 usage in article: APP:IHC, SAMPLE:PANCREATIC TISSUE, DILUTION:1:100

The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlying signaling mechanisms in a rat model of diabetes mellitus

Generation and Phenotype Identification of PAX4 Gene Knockout Rabbit by CRISPR/Cas9 System

Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil?lymphocyte ratio in rats with or without hidden diabetes mellitus

Low dose doxycycline decreases systemic inflammation and improves glycemic control, lipid profiles, and islet morphology and function in db/db mice

Chen Ll, Yang Wh, Zheng J, Hu X, Kong W, Zhang Hh. Nutr Metab (Lond). 2010 May 26;7:45. Doi: 10.1186/1743-7075-7-45. Effect Of Catch-Up Growth After Food Restriction On The Entero-Insular Axis In Rats.

Lan F, Yue X, Han L, Shi Z, Yang Y, Pu P, Yao Z, Kang C. Int J Oncol. 2012 Feb;40(2):519-26. Doi: 10.3892/Ijo.2011.1215. Epub 2011 Sep 28. Genome-Wide Identification Of Tcf7L2/Tcf4 Target Mirnas Reveals A Role For Mir-21 In Wnt-Driven Epithelial C...

Jiang H, Deng Xf, Duan Cm, Chen C, Xiang Jl, Lu Yl, Ma Qf. Lymphology. 2011 Mar;44(1):21-8. Somatostatin Receptors Sstr2 And Sstr5 Are Expressed In The Human Thoracic Duct.

Zhang Y, Ye C, Wang G, Gao Y, Tan K, Zhuo Z, Liu Z, Xia H, Yang D, Li P. Biomed Res Int. 2013;2013:526367. Doi: 10.1155/2013/526367. Epub 2013 May 27. Kidney-Targeted Transplantation Of Mesenchymal Stem Cells By Ultrasound-Targeted Microbubble Des...

Zhang W, Jin H, Hu Y, Yu Y, Li X, Ding Z, Kang Y, Wang B. Hum Gene Ther. 2010 Feb;21(2):171-8. Doi: 10.1089/Hum.2009.095. Protective Response Against Type 1 Diabetes In Nonobese Diabetic Mice After Coimmunization With Insulin And Dna Encoding Proi...

Liu Jj, Raynal S, Bailb?? D, Gausseres B, Carbonne C, Autier V, Movassat J, Kergoat M, Portha B. Biochim Biophys Acta. 2015 May;1852(5):980-91. Doi: 10.1016/J.Bbadis.2015.02.001. Epub 2015 Feb 9. Expression Of The Kynurenine Pathway Enzymes In The...

Tang Mm, Zhu Qe, Fan Wz, Zhang Sl, Li Dz, Liu Lz, Chen M, Zhang M, Zhou J, Wei Cj. Mol Ther. 2011 Jan;19(1):60-6. Doi: 10.1038/Mt.2010.187. Epub 2010 Sep 14. Intra-Arterial Targeted Islet-Specific Expression Of Sirt1 Protects ?? Cells From Strepto...

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5 Customer Q&As for Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10)

Question

Is this MA1047 anti-Glucagon antibody (Monoclonal, K79bB10) reactive to the isotypes of GCG?

Verified Customer

Verified customer

Asked: 2018-06-04

Answer

The immunogen of MA1047 anti-Glucagon antibody (Monoclonal, K79bB10) is Polymerized porcine glucagon. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-06-04

Question

Can you help my question with product MA1047, anti-Glucagon antibody (Monoclonal, K79bB10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-01-11

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1047 anti-Glucagon antibody (Monoclonal, K79bB10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-11

Question

I was wanting to use your anti-Glucagon antibody (Monoclonal, K79bB10) for IHC for rat pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat pancreas identification?

K. Thomas

Verified customer

Asked: 2017-10-09

Answer

It shows on the product datasheet, MA1047 anti-Glucagon antibody (Monoclonal, K79bB10) has been tested for IHC on human, mouse, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pancreas in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-09

Question

Will MA1047 anti-Glucagon antibody (Monoclonal, K79bB10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Kulkarni

Verified customer

Asked: 2016-05-16

Answer

As indicated on the product datasheet, MA1047 anti-Glucagon antibody (Monoclonal, K79bB10) as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-05-16

Question

I have attached the WB image, lot number and protocol we used for pancreas using anti-Glucagon antibody (Monoclonal, K79bB10) MA1047. Please let me know if you require anything else.

J. Jones

Verified customer

Asked: 2014-07-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-07-10

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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MA1047
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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