Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10)

IHC analysis of Glucagon using anti-Glucagon antibody (MA1047).
Glucagon was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Glucagon Antibody (MA1047) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

IHC analysis of Glucagon using anti-Glucagon antibody (MA1047).
Glucagon was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Glucagon Antibody (MA1047) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Effects of the RBPP-P on islet histology. (A) Immunofluorescence (IF) staining for insulin (green) and glucagon (red). Scale bar, 100 μm. (B) β-cell area/pancreatic area ratio, measured by immunofluorescence staining ( n = 5). (C) Insulin levels in serum ( n = 10). All data are expressed as mean ± s. d. # p < 0.05, vs. C57BL/6J mice; *p < 0.05, vs. ALX mice.
Index in PubMed under a CC BY license. PMID: 34262457

IF analysis of Glucagon using anti-Glucagon antibody (MA1047).
Glucagon was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-Glucagon Antibody (MA1047) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.