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Product Info Summary
| SKU: | A02174 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Goat |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HR23B RAD23B Antibody
SKU/Catalog Number
A02174
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-HR23B RAD23B Antibody (Catalog # A02174). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-HR23B RAD23B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02174)
Host
Goat
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 155-180 of human HR23B protein.
Reactive Species
A02174 is reactive to RAD23B in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
43.2 kDa
Background of RAD23B
HR23B (also known as UV excision repair protein RAD23 homolog B, XP-C repair complementing complex 58 kDa protein and p58) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02174 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
WB: 1:500 - 1:2,000
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 58 kDa in size corresponding to HR23B by western blotting in the appropriate cell lysate or extract.
Validation Images & Assay Conditions
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NER mechanism recognizes damaged DNA regions based on their abnormal structure as well as on their abnormal chemistry, then excises and replaces them. The overall process of NER in eukaryotic cells resembles that in E. coli. The initial steps depend on whether the damage is in the actively transcribed strand of a gene or elsewhere in the genome. If the damage is not in the actively transcribed strand of a gene, then the damage is recognized and bound by a heterodimer consisting of the XPC and HR23B proteins. The binding of XPC and HR23B initiates the process of ''global genome repair'' (GGR). The XPC/HR23B dimer appears to recognize damaged DNA based on the extent of distortion of the normal helical DNA structure caused by the damage. In the process of binding to the damaged region, XPC/HR23B is thought to further increase the extent of structural distortion
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Western blot using Boster's affinity purified anti-HR23B antibody shows detection of a band at ~58 kDa (arrowhead) corresponding to HR23B present in a HeLa whole cell lysate . Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data not shown). Approximately 33 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Specific Publications For Anti-HR23B RAD23B Antibody (A02174)
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6 Customer Q&As for Anti-HR23B RAD23B Antibody
Question
Is this A02174 anti-HR23B antibody reactive to the isotypes of RAD23B?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
The immunogen of A02174 anti-HR23B antibody is This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 163-176 of human HR23B protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-05
Question
My question regarding product A02174, anti-HR23B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Banerjee
Verified customer
Asked: 2019-07-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02174 anti-HR23B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-03
Question
I have attached the WB image, lot number and protocol we used for liver using anti-HR23B antibody A02174. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-28
Question
Will A02174 anti-HR23B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Collins
Verified customer
Asked: 2018-09-04
Answer
As indicated on the product datasheet, A02174 anti-HR23B antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-04
Question
We are currently using anti-HR23B antibody A02174 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
H. Jones
Verified customer
Asked: 2017-06-09
Answer
The anti-HR23B antibody (A02174) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-09
Question
you antibody to test anti-HR23B antibody A02174 on human liver for research purposes, then I may be interested in using anti-HR23B antibody A02174 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Moore
Verified customer
Asked: 2015-05-22
Answer
The products we sell, including anti-HR23B antibody A02174, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-05-22
