Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22)

Hspa1b antibody

Boster Bio Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) catalog # MA1050. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rabbit, Rat. Cited in 9 publication(s).

Product Info Summary

SKU: MA1050
Size: 100μg/vial
Reactive Species: Bovine, Human, Mouse, Rabbit, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22)

View all Hspa1b Antibodies

SKU/Catalog Number

MA1050

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) catalog # MA1050. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1050)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

BRM-22

Isotype

Mouse IgG1

Immunogen

HSP70 isolated from bovine brain.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1050 is reactive to Hspa1b in Bovine, Human, Mouse, Rabbit, Rat

Applications

MA1050 is guaranteed for IHC, WB Boster Guarantee

Background of Hspa1b

Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, -
Western blot, 1μg/ml, Human, bovine, mouse, rabbit, rat

Validation Images & Assay Conditions

Gene/Protein Information For Hspa1b (Source: Uniprot.Org, NCBI)

Uniprot ID

P17879

Gene ID

15511

Gene Name

Hspa1b

Full Name

Heat shock 70 kDa protein 1B

Weight

70.176kDa

Superfamily

heat shock protein 70 family

Alternative Names

FLJ54328; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1B; heat shock 70kDa protein 1B; HSP70.1/HSP70.2; HSP70-1/HSP70-2; HSP70-1B; HSP70-2; HSPA1; HSPA1A Hspa1b|HSP70B1, Hsp70, Hsp70-1, Hsp70.1, hsp, hsp68|heat shock protein 1B|heat shock 70 kDa protein 1B|68 kDa heat shock protein|heat shock 70 kDa protein 1|heat shock 70kDa protein 1B|heat shock protein 70.1|heat shock protein, 70 kDa 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Hspa1b, check out the Hspa1b Infographic

Hspa1b infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Hspa1b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1050 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression dynamics of HSP70 during chronic heat stress in Tharparkar cattle

Wang,D.,Ripley-Gonzalez,J.W.& Hu,Y.Aerobic Physical Training Protects the Rat Brain Against Exercise-Heat Related Oxidative Damage through the Increased Expression of HSP70.Neurophysiology 51,66–71(2019).https://doi.org/10.1007/s11062-019-09794-9
Species: Rat
MA1050 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:400

Jing J, Liu H, Chen H, Hu S, Xiao K, Ma X. Chemosphere. 2013 May;91(8):1113-22. Doi: 10.1016/J.Chemosphere.2013.01.014. Epub 2013 Feb 10. Acute Effect Of Copper And Cadmium Exposure On The Expression Of Heat Shock Protein 70 In The Cyprinidae Fish...

Chen S, Zuo X, Yang M, Lu H, Wang N, Wang K, Tu Z, Chen G, Liu M, Liu K, Xiao X. J Leukoc Biol. 2012 Oct;92(4):851-7. Doi: 10.1189/Jlb.0212060. Epub 2012 Jun 29. Severe Multiple Organ Injury In Hsf1 Knockout Mice Induced By Lipopolysaccharide Is A...

Ouyang W, Gao F, Wang L, Xie X, Lei F, Zhou J, Liao Y, Zhong M, Tang J. Oncol Rep. 2010 Oct;24(4):973-9. Thermoseed Hyperthermia Treatment Of Mammary Orthotopic Transplantation Tumors In Rats And Impact On Immune Function.

Xu Xg, Luo Yj, Wu Y, Chen Jz, Xu Th, Gao Xh, He Cd, Geng L, Xiao T, Zhang Yq, Chen Hd, Li Yh. Dermatol Surg. 2011 Aug;37(8):1141-9. Doi: 10.1111/J.1524-4725.2011.02062.X. Epub 2011 Jun 7. Immunohistological Evaluation Of Skin Responses After Treat...

Chen N, Wan Xl, Huang Cx, Wang Wm, Liu H, Wang Hl. Immunobiology. 2014 Nov;219(11):850-8. Doi: 10.1016/J.Imbio.2014.07.012. Epub 2014 Jul 21. Study On The Immune Response To Recombinant Hsp70 Protein From Megalobrama Amblycephala.

Fan Sj, Jiang H, Yang Lj, Liu X, Song J, Pan F. Ann Anat. 2011 Oct 20;193(5):418-24. Doi: 10.1016/J.Aanat.2011.06.001. Epub 2011 Jul 8. Effects Of Adrenergic Agents On Stress-Induced Brain Microstructural And Immunochemical Changes In Adult Male W...

Yi Sx, Peng Y, Chang Xr, Peng N, Yan J, Lin Yp. World J Gastroenterol. 2007 Apr 21;13(15):2174-8. Effect Of Pre-Moxibustion On Apoptosis And Proliferation Of Gastric Mucosa Cells.

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MA1050
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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