Anti-HSP70 Antibody (Monoclonal, BRM-22)

SKU MA1050
Size 100μg/vial
Reactivity Bovine, Human, Rabbit, Rat
Clonality Monoclonal
Clone Number BRM-22
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB


Product Name Anti-HSP70 Antibody (Monoclonal, BRM-22)
SKU/Catalog Number MA1050
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for HSP70, heat shock 70kDa protein 1A; heat shock 70kDa protein 1B (HSPA1A; HSPA1B) detection. Tested with WB, IHC-P, IHC-F in Human;bovine;rabbit;rat. No cross reactivity with other proteins.
Cite This Product Anti-HSP70 Antibody (Monoclonal, BRM-22) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1050)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen HSP70 isolated from bovine brain.
Reactivity Bovine, Human, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, -
Western blot, 0.5μg/ml, Human, bovine, rabbit, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

Boster Kit Box

Target Info

Protein Target Info (Source:

Uniprot Id P17879
Gene Name Hspa1b
Protein Name Heat shock 70 kDa protein 1B
Tissue Specificity Testis-specific.
Alternative Names Heat shock 70 kDa protein 1B;Heat shock 70 kDa protein 1;HSP70.1;Hspa1b;Hcp70.1, Hsp70-1, Hsp70a1, Hspa1;
Subcellular Localization Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
Molecular Weight 70176 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.


Protein Function In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. .
Research Areas Bovine, Human, Rabbit, Rat

*You can search these to find other products in these research areas.
Background Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex(MHC) class III region 92 kilobases(kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.

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Monoclonal clone# G2 antibody for HSPA1A; HSPA1B detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Bovine. HSPA1A; HSPA1B information: Molecular Weight: 70176 MW; Subcellular Localization: Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs; Tissue Specificity: Testis-specific.
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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)



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Jing J, Liu H, Chen H, Hu S, Xiao K, Ma X. Chemosphere. 2013 May;91(8):1113-22. Doi: 10.1016/J.Chemosphere.2013.01.014. Epub 2013 Feb 10. Acute Effect Of Copper And Cadmium Exposure On The Expression Of Heat Shock Protein 70 In The Cyprinidae Fish...
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Xu Xg, Luo Yj, Wu Y, Chen Jz, Xu Th, Gao Xh, He Cd, Geng L, Xiao T, Zhang Yq, Chen Hd, Li Yh. Dermatol Surg. 2011 Aug;37(8):1141-9. Doi: 10.1111/J.1524-4725.2011.02062.X. Epub 2011 Jun 7. Immunohistological Evaluation Of Skin Responses After Treat...
Chen N, Wan Xl, Huang Cx, Wang Wm, Liu H, Wang Hl. Immunobiology. 2014 Nov;219(11):850-8. Doi: 10.1016/J.Imbio.2014.07.012. Epub 2014 Jul 21. Study On The Immune Response To Recombinant Hsp70 Protein From Megalobrama Amblycephala.
Fan Sj, Jiang H, Yang Lj, Liu X, Song J, Pan F. Ann Anat. 2011 Oct 20;193(5):418-24. Doi: 10.1016/J.Aanat.2011.06.001. Epub 2011 Jul 8. Effects Of Adrenergic Agents On Stress-Induced Brain Microstructural And Immunochemical Changes In Adult Male W...
Yi Sx, Peng Y, Chang Xr, Peng N, Yan J, Lin Yp. World J Gastroenterol. 2007 Apr 21;13(15):2174-8. Effect Of Pre-Moxibustion On Apoptosis And Proliferation Of Gastric Mucosa Cells.

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to hsp72 antibody, hspa1b antibody, hsp70i antibody
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