Anti-HSP70 Antibody (Monoclonal, BRM-22)
|Reactivity||Bovine, Human, Rabbit, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-HSP70 Antibody (Monoclonal, BRM-22)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for HSP70, heat shock 70kDa protein 1A; heat shock 70kDa protein 1B (HSPA1A; HSPA1B) detection. Tested with WB, IHC-P, IHC-F in Human;bovine;rabbit;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-HSP70 Antibody (Monoclonal, BRM-22) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1050)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||HSP70 isolated from bovine brain.|
|Reactivity||Bovine, Human, Rabbit, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, -
Western blot, 0.5μg/ml, Human, bovine, rabbit, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||Heat shock 70 kDa protein 1B|
|Alternative Names||Heat shock 70 kDa protein 1B;Heat shock 70 kDa protein 1;HSP70.1;Hspa1b;Hcp70.1, Hsp70-1, Hsp70a1, Hspa1;|
|Subcellular Localization||Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .|
|Molecular Weight||70176 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. .|
|Research Areas||Bovine, Human, Rabbit, Rat
*You can search these to find other products in these research areas.
|Background||Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex(MHC) class III region 92 kilobases(kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact email@example.com
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to firstname.lastname@example.org.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to hsp72 antibody, hspa1b antibody, hsp70i antibody