Click image to see more details
Product Info Summary
| SKU: | MA1050 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Bovine, Human, Mouse, Rabbit, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22)
SKU/Catalog Number
MA1050
BM0368 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) catalog # MA1050. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1050)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
BRM-22
Isotype
Mouse IgG1
Immunogen
HSP70 isolated from bovine brain.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1050 is reactive to Hspa1b in Bovine, Human, Mouse, Rabbit, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
70.2 kDa
Background of Hspa1b
Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1050 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, rabbit, rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, -
Western blot, 1μg/ml, Human, bovine, mouse, rabbit, rat
Positive Control
WB: human MCF-7 whole cell, human 293T whole cell, human A549 whole cell, human U20S whole cell, rat lung tissue, rat liver tissue, mouse lung tissue, mouse liver tissue
IHC: human breast cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HSP70 using anti-HSP70 antibody (MA1050).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSP70 antigen affinity purified monoclonal antibody (Catalog # MA1050) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSP70 at approximately 70 kDa. The expected band size for HSP70 is at 70 kDa.
Click image to see more details
IHC analysis of HSP70 using anti-HSP70 antibody (MA1050).
HSP70 was detected in paraffin-embedded section of human breast cancer tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSP70 Antibody (MA1050) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Specific Publications For Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) (MA1050)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
