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Product Info Summary
| SKU: | M08081 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F |
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Product info
Product Name
Anti-Hu CD112 Purified NECTIN2 Monoclonal Antibody
SKU/Catalog Number
M08081
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD112 Purified NECTIN2 Monoclonal Antibody (Catalog# M08081). Tested in Flow Cytometry, IP, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD112 Purified NECTIN2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08081)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
R2.525
Isotype
Mouse IgG1 kappa
Immunogen
NIH/3T3 cells transfected with human Nectin-2. The mouse monoclonal antibody R2.525 recognizes an extracellular epitope on CD112, a type I transmembrane glycoprotein expressed by myelomonocytic and megakaryocytic cells, and by CD34+ hematopoietic progenitors.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M08081 is reactive to NECTIN2 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
57.7 kDa
Background of NECTIN2
CD112, also known as nectin-2, is a transmembrane glycoprotein involved in organization of adherens junctions. It also serves as a target molecule for entry of certain strains of herpes simplex virus (HSV) and pseudorabies virus (PRV). It is homologous to CD155, which serves as a target molecule for polio virus. CD112 seems to play a role in neural tube formation, with N-cadherin. Inside the cell, CD112 is connected with actin cytoskeleton through afadin. Variations in the CD112 gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08081 is guaranteed for Flow Cytometry, IP, IHC-F Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Separation of human CD112 positive thrombocytes(red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD112 (R2.525) purified antibody (concentration in sample 9 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD112 (R2.525) purified antibody (concentration in sample 9 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD112 Purified NECTIN2 Monoclonal Antibody (M08081)
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