Click image to see more details
Product Info Summary
| SKU: | B05470 |
|---|---|
| Size: | 50ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129
SKU/Catalog Number
B05470
Size
50ug
Form
Liquid
Description
Boster Bio Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129 (Catalog # B05470). Tested in ELISA, ICC, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129 (Boster Biological Technology, Pleasanton CA, USA, Catalog # B05470)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM129
Isotype
Rabbit IgG
Immunogen
Human IgG
Cross-reactivity
This antibody reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
B05470 is reactive to IGKC in Human
Calculated molecular weight
11.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
B05470 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.02 µg/mL – 0.25 µg/mL
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL
Flow Cytometry (FC): 0.5 µg/mL - 2 µg/mL
Validation Images & Assay Conditions
Click image to see more details
ELISA result showing specificity
Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
Click image to see more details
ELISA result showing specificity
ELISA showing RM129 reacts only to kappa and lambda light chain of all human immunoglobulins, not to mouse IgG, rat IgG, or goat IgG.
Click image to see more details
ELISA result showing specificity
ELISA showing RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Click image to see more details
ELISA result showing specificity
A titer Sandwich ELISA using Biotinylated RM129 as the detection antibody. The plate, coated with the capture antibody anti-human IgG RM116, was loaded with different amounts of human IgG. A serial dilution of Biotin RM129 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.
Specific Publications For Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129 (B05470)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
