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Product Info Summary
| SKU: | B05470 |
|---|---|
| Size: | 50ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129
View all Kappa Light Chain Antibodies
SKU/Catalog Number
B05470
Size
50ug
Form
Liquid
Description
Boster Bio Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129 (Catalog # B05470). Tested in ELISA, ICC, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129 (Boster Biological Technology, Pleasanton CA, USA, Catalog # B05470)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM129
Isotype
Rabbit IgG
Immunogen
Human IgG
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
B05470 is reactive to IGKC in Human
Applications
B05470 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
11.765kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.02 µg/mL – 0.25 µg/mL
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL
Flow Cytometry (FC): 0.5 µg/mL - 2 µg/mL
Validation Images & Assay Conditions
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Figure 1. ELISA result showing specificity
Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
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Figure 2. ELISA result showing specificity
ELISA showing RM129 reacts only to kappa and lambda light chain of all human immunoglobulins, not to mouse IgG, rat IgG, or goat IgG.
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Figure 4. ELISA result showing specificity
ELISA showing RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
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Figure 5. ELISA result showing specificity
A titer Sandwich ELISA using Biotinylated RM129 as the detection antibody. The plate, coated with the capture antibody anti-human IgG RM116, was loaded with different amounts of human IgG. A serial dilution of Biotin RM129 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.
Protein Target Info & Infographic
Gene/Protein Information For IGKC (Source: Uniprot.org, NCBI)
Gene Name
IGKC
Full Name
Immunoglobulin kappa constant
Weight
11.765kDa
Alternative Names
HCAK1; IGKCD; immunoglobulin kappa constant; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809 IGKC HCAK1D, Km, IGKC immunoglobulin kappa constant immunoglobulin kappa (invariant region)|immunoglobulin kappa constant region|immunoglobulin kappa light chain (VJ)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGKC, check out the IGKC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGKC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129 (B05470)
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No publications found for B05470
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