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Product Info Summary
| SKU: | M07514-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128
SKU/Catalog Number
M07514-1
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 (Catalog # M07514-1). Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07514-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM128
Isotype
Rabbit IgG
Immunogen
Human IgA
Cross-reactivity
This antibody reacts to human IgA, including both IgA1 and IgA2. No cross-reactivity with human IgG, IgM, IgD, or IgE.
Reactive Species
M07514-1 is reactive to IGHA1 in Human
Calculated molecular weight
37.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07514-1 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection).
Validation Images & Assay Conditions
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IHC result
Immunohistochemistry of human lymphoid tissue using anti-human IgA antibody RM128
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ELISA result showing specificity
Sandwich ELISA using RM128 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA result showing specificity
Sandwich ELISA using RM128 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM128 reacted to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM128. The plate was coated with different amounts of human IgA. A serial dilution of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 (M07514-1)
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