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Product Info Summary
| SKU: | M04575-5 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117
SKU/Catalog Number
M04575-5
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 (Catalog # M04575-5). Tested in ELISA, ICC, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04575-5)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM117
Isotype
Rabbit IgG
Immunogen
Peptide corresponding to the hinge region of Human IgG1
Cross-reactivity
Reacts to the heavy chain of human IgG1. RM117 does not cross-react to any other IgG subclasses (IgG2, IgG3, IgG4), and shows no cross-reactivity to IgM, IgA, IgD, IgE. RM117 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M04575-5 is reactive to IGHG1 in Human
Calculated molecular weight
36.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04575-5 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL
Flow Cytometry (FC): 2ug/mL-8ug/mL.
Validation Images & Assay Conditions
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ELISA result showing specificity
Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
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ELISA result showing specificity
Sandwich ELISA using RM117 as the capture antibody, and Biotinylated anti-human light chains (k+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM117 only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
ELISA showing RM117 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM117 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
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IHC result
Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG1 antibody RM117 at a 1:15,000 dilution.
Specific Publications For Anti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 (M04575-5)
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