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Product Info Summary
| SKU: | M07469-3 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, ICC |
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Product info
Product Name
Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121
SKU/Catalog Number
M07469-3
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 (Catalog # M07469-3). Tested in ELISA, ICC, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07469-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM121
Isotype
Rabbit IgG
Immunogen
Human IgM
Cross-reactivity
This antibody reacts to the heavy chain of human IgM. No cross-reactivity with human IgG, IgA, IgD, or IgE.
Reactive Species
M07469-3 is reactive to IGHM in Human
Calculated molecular weight
49.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07469-3 is guaranteed for ELISA, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Validation Images & Assay Conditions
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ELISA result showing specificity
Sandwich ELISA using RM121 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA result showing specificity
Sandwich ELISA using RM121 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM121 only reacted to human IgM. No cross reactivity with IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM121. The plate was coated with different amounts of human IgM. A serial dilution of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 (M07469-3)
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Customer Q&As
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3 Customer Q&As for Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121
Question
Does M07469-3 cross react with murine IgM?
Verified customer
Asked: 2022-03-06
Answer
No, Our Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 (M07469-3) does not cross react with murine IgM.
Boster Scientific Support
Answered: 2022-03-06
Question
What is the suggested dilution ratio for ICC and IHC of M07469-3?
Verified customer
Asked: 2019-08-12
Answer
For the Anti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone RM121 (M07469-3), the suggested dilution ratio for Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Boster Scientific Support
Answered: 2019-08-12
Question
We are currently using anti-Human IgM Rabbit Monoclonal antibody, Clone#RM121 M07469-3 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
The anti-Human IgM Rabbit Monoclonal antibody, Clone#RM121 (M07469-3) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-14
