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Product Info Summary
| SKU: | M07469-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Mouse IgM Ighm Rabbit Monoclonal Antibody, Clone#RM109
SKU/Catalog Number
M07469-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Mouse IgM Ighm Rabbit Monoclonal Antibody, Clone#RM109 (Catalog # M07469-2). Tested in ELISA applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mouse IgM Ighm Rabbit Monoclonal Antibody, Clone#RM109 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07469-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM109
Isotype
Rabbit IgG
Immunogen
Mouse IgM
Cross-reactivity
This antibody reacts to mouse IgM. No cross-reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG.
Reactive Species
M07469-2 is reactive to Ighm in Mouse
Calculated molecular weight
50.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07469-2 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.005 ug/mL – 0.2 ug/mL.
Validation Images & Assay Conditions
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ELISA result showing specificity
A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of RM109 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
ELISA of mouse immunoglobulins shows RM109 reacted to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM109 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Mouse IgM Ighm Rabbit Monoclonal Antibody, Clone#RM109 (M07469-2)
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