Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody

Product Name

Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody

View all IL25 Antibodies

SKU/Catalog Number

B04429

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody (Catalog # B04429). Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # B04429)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

Clone: 11K8B1 IgG1

Isotype

IgG1

Immunogen

Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

B04429 is reactive to IL25 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

20.3 kDa

Background of IL25

Anti-IL17E recognizes IL-17E (also known as IL25). IL-17E is produced and secreted by intra-epithelial lymphocytes, lung epithelial cells, alveolar macrophages, eosinophils, basophils, NKT cells, mast cells and cells of the gastro-intestinal tract and uterus. IL-17E stimulates the production of Th2-type cytokines. IL-17E limits Th17 cell development by inducing the expression of IL-13 by dendritic cells or by inhibiting IL-23 production by macrophages. Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. A number of cytokines, including IL-17E, have been implicated as oncogenes, reflecting the importance of these proteins in cell growth and survival. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and pro-inflammatory pathologies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

B04429 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
Flow Cytometry: 1µg/mL
Fluorochrome Protein Value: 10-20
WB: 0.5µg/mL
This purified antibody has been tested for use in Flow Cytometry and western blotting. Specific conditions for reactivity should be optimized by the end user.

Validation Images & Assay Conditions

See full target information IL25

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