Click image to see more details
Product Info Summary
| SKU: | A04429-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-IL17E/IL25 Antibody Picoband®
SKU/Catalog Number
A04429-1
Size
100 μg/vial
Form
Lyophilized
Description
IL17E (IL-25) is a cytokine that signals via an IL17RA/IL17RB receptor complex, linking it to IL-17-family signaling modules; UniProt notes IL17RA/IL17RB receptor usage (and associated downstream signaling), and Reactome captures IL-25 dimer binding to IL17RA/IL17RB. Assay context: this Picoband® antibody datasheet specifies WB and ELISA (capture) applications; immunogen is E. coli–derived human IL17E recombinant protein (Y33–G177), with suggested dilutions for WB (0.1–0.5 µg/mL) and ELISA (Cap, 1–5 µg/mL). IL-25 biology is commonly interpreted with other cytokine/chemokine axes such as IL-10 (regulatory) and CCL4 (chemotaxis) in immune profiling panels (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL17E/IL25 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04429-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL17E recombinant protein (Position: Y33-G177).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04429-1 is reactive to IL25 in Human, Mouse, Rat
Observed Molecular Weight
17 kDa
Calculated molecular weight
20.3 kDa
Background of IL25
CAP/Ponsinprotein, also known as Sorbin and SH3 domain-containing protein 1 is a protein that in humans is encoded by the SORBS1 gene. It is mapped to 10q24.1. This gene encodes a CBL-associated protein which functions in the signaling and stimulation of insulin. Mutations in this gene may be associated with human disorders of insulin resistance. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04429-1 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
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Western blot analysis of IL17E using anti-IL17E antibody (A04429-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human IL25 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17E antigen affinity purified polyclonal antibody (Catalog # A04429-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17E at approximately 17KD. The expected band size for IL17E is at 17KD.
Specific Publications For Anti-IL17E/IL25 Antibody Picoband® (A04429-1)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-IL17E/IL25 Antibody Picoband®
Question
I am looking for to test anti-IL17E/IL25 antibody A04429-1 on human testis for research purposes, then I may be interested in using anti-IL17E/IL25 antibody A04429-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The products we sell, including anti-IL17E/IL25 antibody A04429-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-16
Question
Will anti-IL17E/IL25 antibody A04429-1 work for ELISA with testis?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
According to the expression profile of testis, IL25 is highly expressed in testis. So, it is likely that anti-IL17E/IL25 antibody A04429-1 will work for ELISA with testis.
Boster Scientific Support
Answered: 2020-03-03
Question
My question regarding product A04429-1, anti-IL17E/IL25 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04429-1 anti-IL17E/IL25 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-25
Question
Will A04429-1 anti-IL17E/IL25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Bhatt
Verified customer
Asked: 2019-06-04
Answer
You can see on the product datasheet, A04429-1 anti-IL17E/IL25 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-04
Question
Is a blocking peptide available for product anti-IL17E/IL25 antibody (A04429-1)?
A. Thomas
Verified customer
Asked: 2018-10-30
Answer
We do provide the blocking peptide for product anti-IL17E/IL25 antibody (A04429-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-10-30
Question
Does anti-IL17E/IL25 antibody A04429-1 work on zebrafish WB with right atrium auricular region?
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
Our lab technicians have not validated anti-IL17E/IL25 antibody A04429-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IL17E/IL25 antibody A04429-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish right atrium auricular region in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-09
Question
I see that the anti-IL17E/IL25 antibody A04429-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-06-01
Question
Here is the WB image, lot number and protocol we used for testis using anti-IL17E/IL25 antibody A04429-1. Please let me know if you require anything else.
L. Thomas
Verified customer
Asked: 2018-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-06
Question
Is there a BSA free version of anti-IL17E/IL25 antibody A04429-1 available?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL17E/IL25 antibody A04429-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-IL17E/IL25 antibody A04429-1. Let me know if you need anything else.
R. Anderson
Verified customer
Asked: 2017-09-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-20
Question
Is this A04429-1 anti-IL17E/IL25 antibody reactive to the isotypes of IL25?
C. Anderson
Verified customer
Asked: 2017-08-29
Answer
The immunogen of A04429-1 anti-IL17E/IL25 antibody is E. coli-derived human IL17E recombinant protein (Position: Y33-G177). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-29
Question
I was wanting to use your anti-IL17E/IL25 antibody for ELISA for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
V. Thomas
Verified customer
Asked: 2016-05-19
Answer
It shows on the product datasheet, A04429-1 anti-IL17E/IL25 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-19
Question
We are currently using anti-IL17E/IL25 antibody A04429-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
B. Banerjee
Verified customer
Asked: 2016-01-27
Answer
The anti-IL17E/IL25 antibody (A04429-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-01-27
