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Product Info Summary
| SKU: | M12334 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127
SKU/Catalog Number
M12334
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127 (Catalog # M12334). Tested in ELISA, ICC, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12334)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM127
Isotype
Rabbit IgG
Immunogen
Human IgG
Cross-reactivity
This antibody reacts to the lambda light chain of human immunoglobulins, and does not react to the kappa light chain. RM127 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M12334 is reactive to IGLC1 in Human
Calculated molecular weight
11.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12334 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.05ug/mL – 0.2ug/mL
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL
Flow Cytometry (FC): 0.2ug/mL-2ug/mL.
Validation Images & Assay Conditions
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-human Ig lambda light chain rabbit monoclonal antibody (Clone RM127).
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM127 reacted to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM127. The plate was coated with different amounts of human IgG3. A serial dilution of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
ELISA showing RM127 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM127 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Specific Publications For Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127 (M12334)
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Customer Q&As
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1 Customer Q&As for Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127
Question
We are currently using anti-Human Lambda Light Chain Rabbit Monoclonal antibody, Clone#RM127 M12334 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
J. Jones
Verified customer
Asked: 2019-04-16
Answer
The anti-Human Lambda Light Chain Rabbit Monoclonal antibody, Clone#RM127 (M12334) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-16
