Skip to the beginning of the images gallery

IL6 antibody (RP1012) performs well in WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 16 Publications

rp1012 il 6 primary antibodies wb testing 1

Western blot analysis of IL-6 using anti-IL-6 antibody (RP1012).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human IL6 protein 10 ng,
Lane 2: recombinant human IL6 protein 5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-6 antigen affinity purified polyclonal antibody (Catalog # RP1012) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-Interleukin-6 IL6 Antibody Picoband®

Name: Anti-Interleukin-6 IL6 Antibody Picoband®
Brand: Boster Bio
SKU: RP1012
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

IL6 antibody

16 Publications|

1 Review

|Submit a Review

Reactive Species	Host	Application
Human	Rabbit	WB

Product Information

Product Name

Anti-Interleukin-6 IL6 Antibody Picoband®
View all IL6 Antibodies

Form

Lyophilized

Uniprot

P05231

Gene ID

3569

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human IL-6 recombinant protein (Position: P29-M212).

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

20 kDa

Calculated Molecular Weight

23.7 kDa

Background of IL6

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al. (1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al. (1988) mapped the IL-6 gene to chromosome 7p15.

Cite This Product

Anti-Interleukin-6 IL6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1012)

Publications

Geniposidic Acid from Ginger-processed Eucommiae Cortex Alleviates Rheumatoid Arthritis by Modulating Macrophage Lactylation Induced by Exosomes from Inflammatory Fibroblast-Like Synoviocytes

Published: 2025-11-30

Journal: JOURNAL OF NUTRITIONAL BIOCHEMISTRY

Application: WB

Impact Factor: 4.90

Species: Mouse

PMID: 41330460

SKU	RP1012
Application	Western Blot
Sample	human HK-2 cells
Sample Processing Description	Cell samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured at 98 °C. Twenty microliters of each protein sample were loaded per lane onto SDS-PAGE.
Other Reagents	5% non-fat milk
Primary Antibody	Interleukin-6 IL6 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1 h in RT
Detection	Substrate: ECL substrate
Results Summary	Previously, WB experiments were performed using antibodies from two domestic and international suppliers, which showed poor specificity and were expensive. Subsequently, Boster’s IL-6 antibody (Cat. RP1012) was used, demonstrating high specificity, strong titer, cost-effectiveness, and yielding clear bands.

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-Interleukin-6 IL6 Antibody Picoband®

IL6 antibody

Overview

Recommend Dilution

Product Information

Publications

Geniposidic Acid from Ginger-processed Eucommiae Cortex Alleviates Rheumatoid Arthritis by Modulating Macrophage Lactylation Induced by Exosomes from Inflammatory Fibroblast-Like Synoviocytes

Shikonin promotes ferroptosis in HaCaT cells through Nrf2 and alleviates imiquimod-induced psoriasis in mice

Di-(2-ethylhexyl) phthalate aggravates psoriasis-like skin lesions: In vitro and in vivo evaluation

Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways

Circular RNA VMA21 ameliorates sepsis-associated acute kidney injury by regulating miR-9-3p/SMG1/inflammation axis and oxidative stress

MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1

CaSR Antagonist (Calcilytic) NPS 2143 Hinders the Release of Neuroinflammatory IL-6, Soluble ICAM-1, RANTES, and MCP-2 from Aβ-Exposed Human Cortical Astrocytes

miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis

The role of glycogen synthase kinase 3 beta in brain injury induced by myocardial ischemia/reperfusion injury in a rat model of diabetes mellitus

Macrophage Inhibition Factor–Mediated CD74 Signal Modulate Inflammation and Matrix Metabolism in the Degenerated Cartilage Endplate Chondrocytes by Activating Extracellular Signal Regulated Kinase 1/2

The Anti-Lung Cancer Activities of Steroidal Saponins of P. polyphylla Smith var. chinensis (Franch.) Hara through Enhanced Immunostimulation in Experimental Lewis Tumor-Bearing C57BL/6 Mice and Induction of Apoptosis in the A549 Cell Line

Melanocortin 4 receptor antagonists attenuates morphine antinociceptive tolerance, astroglial activation and cytokines expression in the spinal cord of rat

Distribution Change of Mast Cells in Human Nasal Polyps

Expression of the Interleukin 6 System in Cortical Lesions From Patients With Tuberous Sclerosis Complex and Focal Cortical Dysplasia Type IIb

The effects of mifepristone on the expressions of osteopontin, interleukin-6 and leukemia inhibitory factor in the villi of early pregnant women

The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells

Review

Have you used Anti-Interleukin-6 IL6 Antibody Picoband®?

1 Review For Anti-Interleukin-6 IL6 Antibody Picoband®

Using Boster’s IL-6 antibody (RP1012) in WB on HK-2 cells produced clear, specific bands with high sensitivity and cost-effectiveness, outperforming previously tested antibodies.

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality