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Product Info Summary
| SKU: | A04321 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Kif 7 Monoclonal Antibody
SKU/Catalog Number
A04321
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Kif 7 Monoclonal Antibody catalog # A04321. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kif 7 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04321)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
3F8
Isotype
IgG1
Immunogen
Synthetic Peptide
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04321 is reactive to Kif7 in Human, Mouse, Rat
Applications
A04321 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
151.624kDa
Background of Kif7
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTS alpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC, 0.180555555555556
Validation Images & Assay Conditions
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Immunohistochemistry (IHC) staining of Mouse Kidney tissue, diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For Kif7 (Source: Uniprot.org, NCBI)
Gene Name
Kif7
Full Name
Kinesin-like protein KIF7
Weight
151.624kDa
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
EQYK340; kinesin family member 7; kinesin-like protein KIF7; MGC120653; MGC138476; MGC138478; UNQ340 Kif7|kinesin family member 7|kinesin-like protein KIF7|kinesin 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Kif7, check out the Kif7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Kif7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kif 7 Monoclonal Antibody (A04321)
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No publications found for A04321
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-Kif 7 Monoclonal Antibody
Question
Will A04321 anti-Kif 7 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
As indicated on the product datasheet, A04321 anti-Kif 7 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-24
Question
Is this A04321 anti-Kif 7 Monoclonal antibody reactive to the isotypes of KIF7?
C. Zhao
Verified customer
Asked: 2020-02-03
Answer
The immunogen of A04321 anti-Kif 7 Monoclonal antibody is Synthetic Peptide. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-03
Question
See below the WB image, lot number and protocol we used for lung ovary using anti-Kif 7 Monoclonal antibody A04321. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-27
Question
I was wanting to use your anti-Kif 7 Monoclonal antibody for IHC for mouse lung ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung ovary identification?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
As indicated on the product datasheet, A04321 anti-Kif 7 Monoclonal antibody has been validated for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-Kif 7 Monoclonal antibody A04321 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
The anti-Kif 7 Monoclonal antibody (A04321) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-09
Question
Do you have a BSA free version of anti-Kif 7 Monoclonal antibody A04321 available?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Kif 7 Monoclonal antibody A04321 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-14
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung ovary using anti-Kif 7 Monoclonal antibody A04321. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-16
Question
We want to test anti-Kif 7 Monoclonal antibody A04321 on mouse lung ovary for research purposes, then I may be interested in using anti-Kif 7 Monoclonal antibody A04321 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
The products we sell, including anti-Kif 7 Monoclonal antibody A04321, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-04
Question
Will anti-Kif 7 Monoclonal antibody A04321 work on canine IHC with lung ovary?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
Our lab technicians have not tested anti-Kif 7 Monoclonal antibody A04321 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Kif 7 Monoclonal antibody A04321 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine lung ovary in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-01
Question
Is a blocking peptide available for product anti-Kif 7 Monoclonal antibody (A04321)?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
We do provide the blocking peptide for product anti-Kif 7 Monoclonal antibody (A04321). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-25
Question
Would anti-Kif 7 Monoclonal antibody A04321 work for IHC with lung ovary?
N. Brown
Verified customer
Asked: 2016-01-06
Answer
According to the expression profile of lung ovary, KIF7 is highly expressed in lung ovary. So, it is likely that anti-Kif 7 Monoclonal antibody A04321 will work for IHC with lung ovary.
Boster Scientific Support
Answered: 2016-01-06
Question
I have a question about product A04321, anti-Kif 7 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Gonzalez
Verified customer
Asked: 2015-06-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04321 anti-Kif 7 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-06-30
Question
I see that the anti-Kif 7 Monoclonal antibody A04321 works with IHC, what is the protocol used to produce the result images on the product page?
K. Zhang
Verified customer
Asked: 2014-06-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-06-20
