Anti-Plzf/ZBTB16 Antibody Picoband™

Product Name

Anti-Plzf/ZBTB16 Antibody Picoband™

View all PLZF Antibodies

SKU/Catalog Number

PB9969

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Plzf/ZBTB16 Antibody Picoband™ catalog # PB9969. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Plzf/ZBTB16 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9969)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Plzf recombinant protein (Position: M1-E165).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9969 is reactive to ZBTB16 in Human, Rat

Applications

PB9969 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

74 kDa

Calculated molecular weight

74.274kDa

Background of PLZF

Zinc finger and BTB domain-containing protein 16 is a protein that in humans is encoded by the ZBTB16 gene. This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Plzf using anti-Plzf antibody (PB9969).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat ovary tissue lysates,
Lane 2: SKOV3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plzf antigen affinity purified polyclonal antibody (Catalog # PB9969) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plzf at approximately 74 kDa. The expected band size for Plzf is at 74 kDa.

Gene/Protein Information For ZBTB16 (Source: Uniprot.org, NCBI)

Alternative Names

PLZF; PLZFzinc finger and BTB domain-containing protein 16; ZBTB16; zinc finger and BTB domain containing 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; ZNF145; ZNF145Promyelocytic leukemia zinc finger protein ZBTB16 PLZF, ZNF145 zinc finger and BTB domain containing 16 zinc finger and BTB domain-containing protein 16|promyelocytic leukaemia zinc finger|zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia)|zinc finger protein PLZF

For more info on ZBTB16, check out the ZBTB16 Infographic

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In this infographic, you will see the following information for ZBTB16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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