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Product Info Summary
| SKU: | PB9969 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Plzf/ZBTB16 Antibody Picoband®
SKU/Catalog Number
PB9969
PB1010 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Plzf/ZBTB16 Antibody Picoband® catalog # PB9969. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Plzf/ZBTB16 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9969)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Plzf recombinant protein (Position: M1-E165).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9969 is reactive to ZBTB16 in Human, Rat
Observed Molecular Weight
74 kDa
Calculated molecular weight
74.3 kDa
Background of ZBTB16
Zinc finger and BTB domain-containing protein 16 is a protein that in humans is encoded by the ZBTB16 gene. This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9969 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat ovary tissue, SKOV3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Plzf using anti-Plzf antibody (PB9969).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat ovary tissue lysates,
Lane 2: SKOV3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plzf antigen affinity purified polyclonal antibody (Catalog # PB9969) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plzf at approximately 74 kDa. The expected band size for Plzf is at 74 kDa.
Click image to see more details
Relative expression of specific genes in spermatogonial cells. (A) The mRNA expression of TET1 in spermatogonial cells was detected by QRT-PCR. (B) The mRNA expression of PLZF in spermatogonial cells was detected by QRT-PCR. (C) The mRNA expression of GFRα1 in spermatogonial cells was detected by QRT-PCR. (D) The mRNA expression of PRDM1 in spermatogonial cells was detected by QRT-PCR. (E) The mRNA expression of MAGE4 in spermatogonial cells was detected by QRT-PCR. (F) The expression of PLZF in TET1 overexpressed cells was detected by Western Blot. (G) Quantification of PLZF protein levels in TET1 overexpressed cells. (H) The expression of GFRα1 in TET1 overexpressed cells was detected by Western Blot. (I) Quantification of GFRα1 protein levels in TET1 overexpressed cells. p < .0001(****), p < .001(***), p < .01(**), p < .05(*).
Index in PubMed under a CC BY license. PMID: 35222097
Specific Publications For Anti-Plzf/ZBTB16 Antibody Picoband® (PB9969)
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Customer Q&As
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7 Customer Q&As for Anti-Plzf/ZBTB16 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Plzf/ZBTB16 antibody PB9969. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-31
Question
Is this PB9969 anti-Plzf/ZBTB16 antibody reactive to the isotypes of ZBTB16?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The immunogen of PB9969 anti-Plzf/ZBTB16 antibody is E.coli-derived human Plzf recombinant protein (Position: M1-E165). . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-20
Question
Is a blocking peptide available for product anti-Plzf/ZBTB16 antibody (PB9969)?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
We do provide the blocking peptide for product anti-Plzf/ZBTB16 antibody (PB9969). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-16
Question
My lab would like to test anti-Plzf/ZBTB16 antibody PB9969 on human brain for research purposes, then I may be interested in using anti-Plzf/ZBTB16 antibody PB9969 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
The products we sell, including anti-Plzf/ZBTB16 antibody PB9969, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-10
Question
I see that the anti-Plzf/ZBTB16 antibody PB9969 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-11-14
Question
We are currently using anti-Plzf/ZBTB16 antibody PB9969 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on feline tissues as well?
M. Thomas
Verified customer
Asked: 2017-12-14
Answer
The anti-Plzf/ZBTB16 antibody (PB9969) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-14
Question
I have attached the WB image, lot number and protocol we used for brain using anti-Plzf/ZBTB16 antibody PB9969. Please let me know if you require anything else.
N. Parker
Verified customer
Asked: 2014-10-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-15
