Anti-LGI4 Antibody

Product Name

Anti-LGI4 Antibody

View all LGI4 Antibodies

SKU/Catalog Number

A12305

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LGI4 Antibody (Catalog # A12305). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

LGI4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LGI4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12305)

Host

Rabbit

Contents

LGI4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LGI4 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LGI4. The immunogen is located within amino acids 470 - 520 of LGI4.

Cross-reactivity

Two isoforms of LGI4 are known to exist; this LGI4 antibody will recognize only the larger form. This LGI4 antibody is predicted to be specific to LGI4 and not recognize other LGI proteins.

Reactive Species

A12305 is reactive to LGI4 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

59.1 kDa

Background of LGI4

The leucine-rich, glioma inactivated gene 4 (LGI4) is a member of the LGI family in which LGI1 is the exemplar. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 has been identified as a candidate tumor suppressor gene for glioma and plays a role in autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI4 have not been found to be associated with ADTLE. However, the LGI4 gene is located in a region linked to benign familial infantile convulsions. Further study revealed that a GC-to-AT polymorphism was correlated with childhood absence epilepsy. Other studies showed that decreasing LGI4 expression in cultured cells inhibits myelination, indicating that LGI4 may play a role in neural development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A12305 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LGI4 antibody can be used for the detection of LGI4 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information LGI4

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis