Product Info Summary
SKU: | A09516-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MOBP Antibody Picoband®
SKU/Catalog Number
A09516-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MOBP Antibody Picoband® catalog # A09516-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MOBP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09516-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MOBP recombinant protein (Position: Q16-R69). Human MOBP shares 96.2% amino acid (aa) sequence identity with both mouse and rat MOBP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09516-2 is reactive to MOBP in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
20.959kDa
Background of MOBP
MOBP may play a role in compacting or stabilizing the myelin sheath, possibly by binding the negatively charged acidic phospholipids of the cytoplasmic membrane (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09516-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human Caco-2 whole cell, human K562 whole cell, human SH-SY5Y whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
FCM: SH-SY5Y cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MOBP using anti-MOBP antibody (A09516-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOBP antigen affinity purified polyclonal antibody (Catalog # A09516-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOBP at approximately 21 kDa. The expected band size for MOBP is at 21 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-MOBP antibody (A09516-2).
Overlay histogram showing SH-SY5Y cells stained with A09516-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOBP Antibody (A09516-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MOBP (Source: Uniprot.org, NCBI)
Gene Name
MOBP
Full Name
Myelin-associated oligodendrocyte basic protein
Weight
20.959kDa
Alternative Names
MGC87379; myelin-associated oligodendrocyte basic protein MOBP myelin associated oligodendrocyte basic protein myelin-associated oligodendrocyte basic protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MOBP, check out the MOBP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MOBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MOBP Antibody Picoband® (A09516-2)
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