RPS27 antibody (PB10093) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 1 Publication
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Anti-MPS1/RPS27 Antibody Picoband®

RPS27 antibody

Reactive SpeciesHostApplication
Human, Mouse, RatRabbitFlow Cytometry, IF, IHC, IHC-F, ICC, WB

SKU: PB10093

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

1 in stock. Ships Next Business Day.

Quantity: 1

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Tested Applications

RPS27 antibody was positively detected by WB in:

human Caco-2 whole cell, human RT4 whole cell, human Jurkat whole cell, rat PC-12 whole cell, rat RH35 whole cell, mouse RAW2647 whole cell, mouse SP2/0 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

RPS27 antibody was positively detected by IHC in:

human intestinal cancer tissue, mouse brain cancer tissue, rat brain cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

RPS27 antibody was positively detected by ICC/IF in:

U20S cell

RPS27 antibody was positively detected by IF in:

human ovarian cancer tissue

RPS27 antibody was positively detected by FC in:

HeLa cell

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human
Immunocytochemistry
0.5-1μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Reactive Data

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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted

Product Information

Product Name
Anti-MPS1/RPS27 Antibody Picoband®
View all RPS27 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MPS1 recombinant protein (Position: P2-H84). Human MPS1 shares 100% amino acid (aa) sequence identity with both mouse and rat MPS1.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
10 kDa
Calculated Molecular Weight

9.5 kDa

Background of RPS27
40S ribosomal protein S27, also known as metallopan-stimulin 1 or MPS-1, is a protein that in humans is encoded by the RPS27 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Cite This Product
Anti-MPS1/RPS27 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10093)

Protocols

Western Blot protocol for RPS27 antibody
Immunohistochemistry (IHC) protocol for RPS27 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for RPS27 antibody
Immunofluorescence (IF) protocol for RPS27 antibody
Flow Cytometry (FC) protocol for RPS27 antibody

Publications

Application:
Species:
Neuronal Subtype–Specific Ribosomal Protein mRNA Expression
Published: 2026-04-09
Journal: RNA
Application: IF
Impact Factor: 5.00
Species: Mouse
PMID: 41956739

Review

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Product Promise

We promise Anti-MPS1/RPS27 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
PB10093
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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Boster Guarantee

Guaranteed product quality

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 5, 2026