Anti-MPS1/RPS27 Antibody Picoband™

Ribosomal protein S27 antibody

Boster Bio Anti-MPS1/RPS27 Antibody Picoband™ catalog # PB10093. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB10093
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-MPS1/RPS27 Antibody Picoband™

View all Ribosomal protein S27 Antibodies

SKU/Catalog Number

PB10093

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MPS1/RPS27 Antibody Picoband™ catalog # PB10093. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MPS1/RPS27 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10093)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human MPS1 recombinant protein (Position: P2-H84). Human MPS1 shares 100% amino acid (aa) sequence identity with both mouse and rat MPS1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB10093 is reactive to RPS27 in Human, Mouse, Rat

Applications

PB10093 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

10 kDa

Calculated molecular weight

9.461kDa

Background of Ribosomal protein S27

40S ribosomal protein S27, also known as metallopan-stimulin 1 or MPS-1, is a protein that in humans is encoded by the RPS27 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For RPS27 (Source: Uniprot.org, NCBI)

Gene Name

RPS27

Full Name

40S ribosomal protein S27

Weight

9.461kDa

Superfamily

eukaryotic ribosomal protein eS27 family

Alternative Names

Metallopan-stimulin 1; MPS140S ribosomal protein S27; MPS-1metallopanstimulin 1; ribosomal protein S27 (metallopanstimulin 1); ribosomal protein S27 RPS27 DBA17, MPS-1, MPS1, S27 ribosomal protein S27 40S ribosomal protein S27|metallopan-stimulin 1|metallopanstimulin 1|small ribosomal subunit protein eS27

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RPS27, check out the RPS27 Infographic

RPS27 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RPS27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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2 Customer Q&As for Anti-MPS1/RPS27 Antibody Picoband™

Question

Are A05949-1 and PB10093 suitable for intracellular flow cytometry experiments?

Verified customer

Asked: 2022-06-06

Answer

Yes, the Anti-RPL29 Antibody Picoband™ (A05949-1) and Anti-MPS1/RPS27 Antibody Picoband™ (PB10093) are suitable for intracellular flow cytometry experiments.

Boster Scientific Support

Answered: 2022-06-07

Question

We are currently using anti-MPS1/RPS27 antibody PB10093 for human tissue, and we are happy with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

E. Li

Verified customer

Asked: 2017-03-20

Answer

The anti-MPS1/RPS27 antibody (PB10093) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-03-20

Order DetailsPrice
PB10093

100μg

$370
PB10093-10ug

10μg sample (liquid)

$99
PB10093-Biotin

100 μg Biotin conjugated

$570
PB10093-Cy3

100 μg Cy3 conjugated

$570
PB10093-Dylight488

100 μg Dylight488 conjugated

$570
PB10093-Dylight550

100 μg Dylight550 conjugated

$570
PB10093-Dylight594

100 μg Dylight594 conjugated

$570
PB10093-FITC

100 μg FITC conjugated

$570
PB10093-HRP

100 μg HRP conjugated

$570
PB10093-APC

100 μg APC conjugated

$670
PB10093-PE

100 μg PE conjugated

$670
PB10093-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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