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Product Info Summary
| SKU: | A12643 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MRP-S12 Antibody
SKU/Catalog Number
A12643
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRP-S12 Antibody catalog # A12643. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-S12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12643)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS12. AA range:21-70
Reactive Species
A12643 is reactive to MRPS12 in Human, Mouse
Calculated molecular weight
15.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12643 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Western Blot analysis of SH-SY5Y cells using MRP-S12 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of the lysates from HepG2 cells using MRPS12 antibody.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-MRP-S12 Antibody (A12643)
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Customer Q&As
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5 Customer Q&As for Anti-MRP-S12 Antibody
Question
Will A12643 anti-MRP-S12 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
It shows on the product datasheet, A12643 anti-MRP-S12 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-27
Question
Would anti-MRP-S12 antibody A12643 work for WB with liver?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
According to the expression profile of liver, MRPS12 is highly expressed in liver. So, it is likely that anti-MRP-S12 antibody A12643 will work for WB with liver.
Boster Scientific Support
Answered: 2020-02-27
Question
We are currently using anti-MRP-S12 antibody A12643 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The anti-MRP-S12 antibody (A12643) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-25
Question
Is there a BSA free version of anti-MRP-S12 antibody A12643 available?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MRP-S12 antibody A12643 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-30
Question
I was wanting to use your anti-MRP-S12 antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2018-05-21
Answer
As indicated on the product datasheet, A12643 anti-MRP-S12 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-21
