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- Table of Contents
MRPL12 antibody (A10395-3) performs well in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of MRPL12 using anti-MRPL12 antibody (A10395-3).
MRPL12 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-MRPL12 Antibody (A10395-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
MRPL12 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-MRPL12 Antibody (A10395-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-MRPL12 Antibody
MRPL12 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
MRPL12 antibody was positively detected by WB in:
human COLO-320 whole cell, human Hela whole cell, human MCF-7 whole cell, human 293T whole cell, mouse small intestine tissue, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MRPL12 antibody was positively detected by IHC in:
human colon cancer tissue, human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunohistochemistry
1:50-400
Immunocytochemistry/Immunofluorescence
1:50-400
Immunoprecipitation
1:50
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
1:100-1000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
ELISA
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-MRPL12 Antibody
View all MRPL12 Antibodies
View all MRPL12 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human MRPL12 recombinant protein (Position: M1-T194).
Observed Molecular Weight
21 kDa
Calculated Molecular Weight
21.3 kDa
Background of MRPL12
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
Cite This Product
Anti-MRPL12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10395-3)
Protocols
Western Blot protocol for MRPL12 antibody
Immunohistochemistry (IHC) protocol for MRPL12 antibody
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Product Promise
We promise Anti-MRPL12 Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A10395-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026