Anti-Ms CD3 Purified Cd3e Monoclonal Antibody

Product Name

Anti-Ms CD3 Purified Cd3e Monoclonal Antibody

View all CD3E Antibodies

SKU/Catalog Number

M02675

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ms CD3 Purified Cd3e Monoclonal Antibody (Catalog# M02675). Tested in Flow Cytometry, IP, IHC-F, ICC application(s). This antibody reacts with Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Ms CD3 Purified Cd3e Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02675)

Host

Hamster

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

145-2C11

Isotype

Armenian Hamster IgG kappa

Immunogen

Mouse BM10-37 cytotoxic T lymphocytes. The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.

Reactive Species

M02675 is reactive to CD3E in Mouse

Calculated molecular weight

21.4 kDa

Background of CD3E

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02675 is guaranteed for Flow Cytometry, IP, IHC-F, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-2 μg / ml (million cells).
Immunoprecipitation: 1-2 μg / 100-500 μg protein in 1 ml of a cell lysate.

Validation Images & Assay Conditions

See full target information CD3E

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