|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-MEK7 Antibody|
|Description||Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 7(MAP2K7) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-MEK7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1922)|
|Replacement Item||This antibody may replace the following items: sc-13071|sc-15361|sc-25288|sc-7028|sc-7103|sc-7104 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human MEK7(24-40aa ARRRIDLNLDISPQRPR), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Dual specificity mitogen-activated protein kinase kinase 7|
|Molecular Weight||47485 MW|
|Protein Function||Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. .|
|Tissue Specificity||Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.|
|Subcellular Localization||Nucleus. Cytoplasm .|
|Alternative Names||Dual specificity mitogen-activated protein kinase kinase 7;MAP kinase kinase 7;MAPKK 7;184.108.40.206;JNK-activating kinase 2;MAPK/ERK kinase 7;MEK 7;Stress-activated protein kinase kinase 4;SAPK kinase 4;SAPKK-4;SAPKK4;c-Jun N-terminal kinase kinase 2;JNK kinase 2;JNKK 2;MAP2K7;JNKK2, MEK7, MKK7, PRKMK7, SKK4;|
|Research Areas|||immunology|innate immunity|macrophage / inflamm.| signal transduction|protein phosphorylation|ser / thr kinases|mapk pathway| immunology|tlr signaling| metabolism|pathways and processes|redox metabolism|oxidative stress||
Background for Dual specificity mitogen-activated protein kinase kinase 7
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MEK7 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MEK7 Antibody Images
Click the images to enlarge.
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,