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- Table of Contents
ALDH1A1 antibody (A01392) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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9. Flow Cytometry analysis of HL-60 cells using anti-ALDH1A1 antibody (A01392).
Overlay histogram showing HL-60 cells stained with A01392 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1A1 Antibody (A01392,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing HL-60 cells stained with A01392 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1A1 Antibody (A01392,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband®
ALDH1A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ALDH1A1 antibody was positively detected by WB in:
human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP)human HepG2 whole cell, human A549 whole cell, rat liver tissue, rat kidney tissue, mouse lung tissue, human A549- WT whole cell lysates, human A549-ALDH1A1 KO whole cell lysates
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ALDH1A1 antibody was positively detected by IHC in:
human renal cancer tissue, human renal cancer tissue, human mammary cancer tissue, mouse gaster tissue, rat gaster tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ALDH1A1 antibody was positively detected by ICC/IF in:
A431 cell, A549 cell
ALDH1A1 antibody was positively detected by FC in:
HL-60 cell, HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband®
View all ALDH1A1 Antibodies
View all ALDH1A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ALDH1A1 recombinant protein (Position: T6-Q342).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
54.9 kDa
Background of ALDH1A1
Aldehyde dehydrogenase 1 family, member A1, also known as ALDH1A1 or retinaldehyde dehydrogenase 1 (RALDH1), is an enzyme that in humans is encoded by the ALDH1A1 gene. It is mapped to 9q21.13. The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
Cite This Product
Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01392)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
91%
High Homology
Sheep
91%
High Homology
Cow
91%
High Homology
Dog
88%
Moderate Homology
Mouse
87%
Moderate Homology
Rat
86%
Moderate Homology
Zebrafish
73%
Moderate Homology
Drosophila
65%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ALDH1A1 antibody
Immunohistochemistry (IHC) protocol for ALDH1A1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ALDH1A1 antibody
Flow Cytometry (FC) protocol for ALDH1A1 antibody
Publications
Application:
Species:
Dioscin Suppresses Ovarian and Gastric Tumor Progression by Inhibiting ALDH1A3-Mediated Retinoic Acid Metabolism and Cancer Stemness
Published: 2026-02-05
Journal: PHYTOTHERAPY RESEARCH
Application: WB
Impact Factor: 6.30
Species: Human
PMID: 41644120
Propofol Inhibits the Stem Characteristics and Tumor Growth of NSCLC by Altering the Expression and Localization of HDAC1
Published: 2025-12-22
Journal: FASEB JOURNAL
Application: IHC
Impact Factor: 4.20
Species: Mouse
PMID: 41427950
Compound Nanoemulsion Combined with Differentiation/Cytotoxicity Drugs for Modulating Breast Cancer Stemness
Published: 2023-01-30
Journal: MOLECULAR PHARMACEUTICS
Application: WB
Impact Factor: 4.90
Species: Mouse
PMID: 36715483
Retraction of “Label-Free Isolation of Low-Adhesion Cells with Stem Properties for Cancer Stem Cell-Specific Drug Evaluation”
Published: 2022-09-19
Journal: ANALYTICAL CHEMISTRY
Application: WB
Impact Factor: 8.01
Species: Human
PMID: 36122350
A neutrophil-biomimic platform for eradicating metastatic breast cancer stem-like cells by redox microenvironment modulation and hypoxia-triggered differentiation therapy
Published: 2022-05-29
Journal: Acta Pharmaceutica Sinica B
Application: WB
Impact Factor: 14.90
Species: Mouse
PMID: 36815033
Disulfiram/Copper Induces Antitumor Activity against Both Nasopharyngeal Cancer Cells and Cancer-Associated Fibroblasts through ROS/MAPK and Ferroptosis Pathways
Published: 2020-01-06
Journal: Cancers
Application: WB
Impact Factor: 6.13
Species: Mouse
PMID: 31935835
Anti-colorectal cancer biotargets and biological mechanisms of puerarin: Study of molecular networks
Published: 2019-06-21
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IHC
Impact Factor: 3.17
Species: Human
PMID: 31233753
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Product Promise
We promise Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01392
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026