Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody

Boster Bio Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody catalog # A01392. Tested in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A01392
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody

See all Aldehyde Dehydrogenase 1-A1/ALDH1A1 products

SKU/Catalog Number

A01392

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody catalog # A01392. Tested in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01392)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human ALDH1A1 recombinant protein (Position: T6-Q342).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01392 is reactive to ALDH1A1 in Human, Mouse, Rat

Applications

A01392 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ALDH1A1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P00352

Gene ID

216

Gene Name

ALDH1A1

Full Name

Retinal dehydrogenase 1

Weight

54.862kDa

Superfamily

aldehyde dehydrogenase family

Alternative Names

acetaldehyde dehydrogenase 1; ALDC; aldehyde dehydrogenase 1 family, member A1; aldehyde dehydrogenase 1, soluble; Aldehyde Dehydrogenase 1A1; Aldehyde Dehydrogenase 1-A1; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; aldehyde dehydrogenase, liver cytosolic; ALDH class 1; ALDH1; ALDH11; ALDH1A1; ALDH-E1; ALHDII; EC 1.2.1; EC 1.2.1.36; MGC2318; PUMB1; RALDH 1; RALDH1; retinal dehydrogenase 1; retinaldehyde dehydrogenase 1 ALDH1A1 ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1 aldehyde dehydrogenase 1 family member A1 retinal dehydrogenase 1|ALDH class 1|ALHDII|RALDH 1|acetaldehyde dehydrogenase 1|aldehyde dehydrogenase 1, soluble|aldehyde dehydrogenase, liver cytosolic|epididymis luminal protein 12|epididymis luminal protein 9|epididymis secretory sperm binding protein Li 53e|retinaldehyde dehydrogenase 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ALDH1A1, check out the ALDH1A1 Infographic

ALDH1A1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ALDH1A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01392 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li Y,Chen F,Chen J,Chan S,He Y,Liu W,Zhang G.Disulfiram/Copper Induces Antitumor Activity against Both Nasopharyngeal Cancer Cells and Cancer-Associated Fibroblasts through ROS/MAPK and Ferroptosis Pathways.Cancers (Basel).2020 Jan 6;12(1):138.doi:10.3390
Species: Human,Mouse
A01392 usage in article: APP:WB, SAMPLE:NPC CELL LINES AND NP69 CELL, DILUTION:1:100

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5 Customer Q&As for Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody

Question

We need using your anti-ALDH1A1 antibody for cellular aldehyde metabolic process studies. Has this antibody been tested with western blotting on liver tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

Thanks for your inquiry. This A01392 anti-ALDH1A1 antibody is tested on human placenta tissue, mouse lung tissue, rat lung tissue, a549 whole cell lysates, hepg2 whole cell lysates, liver tissue, kidney tissue. It is guaranteed to work for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-09-17

Question

We are currently using anti-ALDH1A1 antibody A01392 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

C. Williams

Verified customer

Asked: 2019-03-19

Answer

The anti-ALDH1A1 antibody (A01392) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-03-19

Question

Our lab were satisfied with the WB result of your anti-ALDH1A1 antibody. However we have seen positive staining in nasal cavity epithelium cytosol using this antibody. Is that expected? Could you tell me where is ALDH1A1 supposed to be expressed?

B. Wu

Verified customer

Asked: 2018-09-19

Answer

Based on literature, nasal cavity epithelium does express ALDH1A1. Generally ALDH1A1 expresses in cytoplasm, cytosol. Regarding which tissues have ALDH1A1 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Erythrocyte, Pubmed ID: 2776714, 19608861
Lens, Pubmed ID: 19296407
Liver, Pubmed ID: 2987944, 6723659, 8214422, 24275569

Boster Scientific Support

Answered: 2018-09-19

Question

We ordered your anti-ALDH1A1 antibody for ICC on nasal cavity epithelium in the past. I am using mouse, and We want to use the antibody for Flow Cytometry next. you antibody examining nasal cavity epithelium as well as erythrocyte in our next experiment. Could give a recommendation on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2017-12-28

Answer

I viewed the website and datasheets of our anti-ALDH1A1 antibody and it seems that A01392 has been tested on mouse in both ICC and Flow Cytometry. Thus A01392 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-12-28

Question

We have been able to see staining in mouse liver. What should we do? Is anti-ALDH1A1 antibody supposed to stain liver positively?

B. Taylor

Verified customer

Asked: 2015-02-19

Answer

According to literature liver does express ALDH1A1. According to Uniprot.org, ALDH1A1 is expressed in nasal cavity epithelium, liver, lens, colon, erythrocyte, among other tissues. Regarding which tissues have ALDH1A1 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Erythrocyte, Pubmed ID: 2776714, 19608861
Lens, Pubmed ID: 19296407
Liver, Pubmed ID: 2987944, 6723659, 8214422, 24275569

Boster Scientific Support

Answered: 2015-02-19

Size

Total: $315

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A01392
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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