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- Table of Contents
MAPK1 antibody (PA1049) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Flow Cytometry analysis of Hela cells using anti-MAPK1/3 antibody (PA1049).
Overlay histogram showing Hela cells stained with PA1049 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAPK1/3 Antibody (PA1049, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Hela cells stained with PA1049 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAPK1/3 Antibody (PA1049, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-MAPK1/3 Antibody Picoband®
MAPK1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, ICC, WB |
Tested Applications
MAPK1 antibody was positively detected by WB in:
human Hela whole cell, human K562 whole cell, human A431 whole cell, human U2OS whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MAPK1 antibody was positively detected by ICC/IF in:
U2OS cell
MAPK1 antibody was positively detected by FC in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Rat
Tested
Mouse
Tested
Rabbit
Predicted
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Monkey
Predicted
Zebrafish
Predicted
Product Information
Product Name
Anti-MAPK1/3 Antibody Picoband®
View all MAPK1 Antibodies
View all MAPK1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MAPK1/3, identical to the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38, 42 kDa
Calculated Molecular Weight
41.4 kDa
Background of MAPK1
MAPK1 (ERK2) shares high homology with MAPK3 (ERK1). MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Mitogen-activated protein (MAP) kinases [also known as Erks] have been established to function as important mediators of signal transduction by growth factor receptors. ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2.
Cite This Product
Anti-MAPK1/3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1049)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
100%
High Homology
Drosophila
86%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MAPK1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MAPK1 antibody
Flow Cytometry (FC) protocol for MAPK1 antibody
Publications
Application:
Species:
Molecular Mechanisms Associated with Protecting IEC-6 Cells from Acrylamide-Induced Tight Junction Damage by Ganoderma atrum Polysaccharide
Published: 2022-12-24
Journal: MOLECULAR NUTRITION & FOOD RESEARCH
Application: WB
Impact Factor: 6.58
Species: Rat
PMID: 36565056
The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells
Published: 2019-09-30
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: WB
Impact Factor: 4.52
Species: Rat
PMID: 31566725
Propionate relieves pentylenetetrazol-induced seizures, consequent mitochondrial disruption, neuron necrosis and neurological deficits in mice
Published: 2019-09-03
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 4.83
Species: Mouse
PMID: 31491413
Autophagy suppresses isoprenaline-induced M2 macrophage polarization via the ROS/ERK and mTOR signaling pathway
Published: 2017-06-21
Journal: FREE RADICAL BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 5.61
PMID: 28647611
Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB/MAPK Signaling Pathway
Published: 2017-06-06
Journal: Evidence-based Complementary and Alternative Medicine
Application: WB
Impact Factor: 1.74
Species: Mouse
PMID: 28676833
Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways
Published: 2016-07-26
Journal: JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
Application: WB
Impact Factor: 4.36
Species: Mouse
PMID: 27456341
Fructus phyllanthi tannin fraction induces apoptosis and inhibits migration and invasion of human lung squamous carcinoma cells in vitro via MAPK/MMP pathways
Published: 2015-04-13
Journal: ACTA PHARMACOLOGICA SINICA
Application: WB
Impact Factor: 2.91
Species: Human
PMID: 25864648
Recombinant Human Kallistatin Inhibits Angiogenesis by Blocking VEGF Signaling Pathway
Published: 2013-10-15
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 3.06
Species: Human
PMID: 24129914
Nitric oxide inhibits gastric cancer cell growth through the modulation of the Akt pathway
Published: 2011-07-15
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 0.31
Species: Human
PMID: 21769431
Treatment of Atherosclerosis by Transplantation of Bone Endothelial Progenitor Cells Over-Expressed Paraoxonase-1 Gene by Recombinant Adeno-Associated Virus in Rat
Published: 2010-11-01
Journal: BIOLOGICAL & PHARMACEUTICAL BULLETIN
Application: IHC
Impact Factor: 1.81
Species: Rat
PMID: 21048304
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Product Promise
We promise Anti-MAPK1/3 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1049
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026