Anti-Ms CD5 Purified Monoclonal Antibody

Product Name

Anti-Ms CD5 Purified Monoclonal Antibody

View all CD5 Antibodies

SKU/Catalog Number

M00480-3

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ms CD5 Purified Monoclonal Antibody (Catalog# M00480-3). Tested in Flow Cytometry, IP, IHC-P, IHC-F application(s). This antibody reacts with Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Ms CD5 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00480-3)

Host

Rat

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

53-7.3

Isotype

Rat IgG2a kappa

Immunogen

Murine thymus or spleen cells. The rat monoclonal antibody 53-7.3 recognizes an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B-1 lymphocytes.

Reactive Species

M00480-3 is reactive to CD5 in Mouse

Observed Molecular Weight

19 kDa

Calculated molecular weight

53.8 kDa

Background of CD5

CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00480-3 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

See full target information CD5

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