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Product Info Summary
| SKU: | PA1424 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interleukin-4 IL4 Antibody Picoband®
SKU/Catalog Number
PA1424
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-4 IL4 Antibody catalog # PA1424. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-4 IL4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1424)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human IL-4.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1424 is reactive to IL4 in Human
Observed Molecular Weight
15 kDa
Calculated molecular weight
17.5 kDa
Background of IL4
Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1424 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-IL-4 antibody, PA1424, Western blotting
Lane 1: Recombinant Human IL-4 Protein 10ng
Lane 2: Recombinant Human IL-4 Protein 5ng
Lane 3: Recombinant Human IL-4 Protein 2.5ng
Specific Publications For Anti-Interleukin-4 IL4 Antibody Picoband® (PA1424)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Interleukin-4 IL4 Antibody Picoband®
Question
We have been able to see staining in human oocyte. Do you have any suggestions? Is anti-IL4 antibody supposed to stain oocyte positively?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
Based on literature oocyte does express IL4. Based on Uniprot.org, IL4 is expressed in oocyte, blood, among other tissues. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-01
Question
Will anti-IL4 antibody PA1424 work for WB with oocyte?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
According to the expression profile of oocyte, IL4 is highly expressed in oocyte. So, it is likely that anti-IL4 antibody PA1424 will work for WB with oocyte.
Boster Scientific Support
Answered: 2019-11-18
Question
We are currently using anti-IL4 antibody PA1424 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
The anti-IL4 antibody (PA1424) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-30
Question
I was wanting to use your anti-IL4 antibody for WB for human oocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human oocyte identification?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
As indicated on the product datasheet, PA1424 anti-IL4 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-19
Question
Can you help my question with product PA1424, anti-IL4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Jones
Verified customer
Asked: 2019-09-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1424 anti-IL4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-16
Question
My team were content with the WB result of your anti-IL4 antibody. However we have observed positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
From what I have seen in literature, blood does express IL4. Generally IL4 expresses in secreted. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-22
Question
We are interested in to test anti-IL4 antibody PA1424 on human oocyte for research purposes, then I may be interested in using anti-IL4 antibody PA1424 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The products we sell, including anti-IL4 antibody PA1424, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-09
Question
Do you have a BSA free version of anti-IL4 antibody PA1424 available?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL4 antibody PA1424 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-27
Question
Is a blocking peptide available for product anti-IL4 antibody (PA1424)?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
We do provide the blocking peptide for product anti-IL4 antibody (PA1424). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-03
Question
I see that the anti-IL4 antibody PA1424 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-10-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oocyte using anti-IL4 antibody PA1424. Let me know if you need anything else.
R. Dhar
Verified customer
Asked: 2016-08-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-29
Question
Is this PA1424 anti-IL4 antibody reactive to the isotypes of IL4?
Z. Baker
Verified customer
Asked: 2015-11-05
Answer
The immunogen of PA1424 anti-IL4 antibody is A synthetic peptide corresponding to a sequence in the middle region of human IL-4(72-98aa AATVLRQFYSHHEKDTR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-05
Question
Does anti-IL4 antibody PA1424 work on goat WB with oocyte?
L. Miller
Verified customer
Asked: 2013-10-02
Answer
Our lab technicians have not validated anti-IL4 antibody PA1424 on goat. You can run a BLAST between goat and the immunogen sequence of anti-IL4 antibody PA1424 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat oocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-02
Question
Please see the WB image, lot number and protocol we used for oocyte using anti-IL4 antibody PA1424. Please let me know if you require anything else.
O. Evans
Verified customer
Asked: 2013-07-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-08
Question
Does PA1424 anti-IL4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Evans
Verified customer
Asked: 2013-01-31
Answer
You can see on the product datasheet, PA1424 anti-IL4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-31
