Anti-IL4 Antibody Picoband™

Boster Bio Anti-IL4 Antibody Picoband™ catalog # A00230. Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: A00230
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-IL4 Antibody Picoband™

See all IL-4 products

SKU/Catalog Number

A00230

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL4 Antibody Picoband™ catalog # A00230. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00230)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human IL4 recombinant protein (Position: H25-S153).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00230 is reactive to IL4 in Human

Applications

A00230 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For IL4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P05112

Gene ID

3565

Gene Name

IL4

Full Name

Interleukin-4

Weight

17.492kDa

Superfamily

IL-4/IL-13 family

Alternative Names

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra IL4 BCGF-1, BCGF1, BSF-1, BSF1, IL-4 interleukin 4 interleukin-4|B cell growth factor 1|B_cell stimulatory factor 1|binetrakin|interleukin 4 variant 2|lymphocyte stimulatory factor 1|pitrakinra

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL4, check out the IL4 Infographic

IL4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00230 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of IFN-γ and IL-4 in the esophageal mucosa of patients with reflux esophagitis and Barrett's esophagus and their relationship with endoscopic and histologic grading

Upregulated protein arginine methyltransferase 1 by IL-4 increases eotaxin-1 expression in airway epithelial cells and participates in antigen-induced pulmonary %u2026

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

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4 Customer Q&As for Anti-IL4 Antibody Picoband™

Question

Our lab used your anti-IL4 antibody for ELISA on oocyte last year. I am using human, and I plan to use the antibody for WB next. We are interested in examining oocyte as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

I looked at the website and datasheets of our anti-IL4 antibody and I see that A00230 has been validated on human in both ELISA and WB. Thus A00230 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-16

Question

We have observed staining in human blood. What should we do? Is anti-IL4 antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

From literature blood does express IL4. From Uniprot.org, IL4 is expressed in oocyte, blood, among other tissues. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-04

Question

We are currently using anti-IL4 antibody A00230 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-02-14

Answer

The anti-IL4 antibody (A00230) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-02-14

Question

Our team were well pleased with the WB result of your anti-IL4 antibody. However we have observed positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL4 supposed to be expressed?

B. Dhar

Verified customer

Asked: 2017-03-03

Answer

From what I have seen in literature, blood does express IL4. Generally IL4 expresses in secreted. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-03-03

Size

Total: $240

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A00230
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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