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Product Info Summary
| SKU: | A00230 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL4 Antibody Picoband®
SKU/Catalog Number
A00230
Size
100 μg/vial
Form
Lyophilized
Description
Cytokine-mediated signaling; immunomodulatory IL-4 contexts in cancer and immune regulation (per product background). Assay: rabbit polyclonal primary validated for WB/ELISA; reactive species Human; observed MW ~20 kDa; immunogen E. coli-derived human IL-4 (H25–S153). Often profiled with inflammatory markers such as IL-6 and TNF-α to contextualize immune milieu (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00230)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL4 recombinant protein (Position: H25-S153).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00230 is reactive to IL4 in Human
Observed Molecular Weight
20 kDa
Calculated molecular weight
17.5 kDa
Background of IL4
Interleukin-4 (IL-4), also known as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00230 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human CCRF-CEM whole cell
Validation Images & Assay Conditions
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Western blot analysis of IL4 using anti-IL4 antibody (A00230).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human CCRF-CEM whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL4 antigen affinity purified polyclonal antibody (Catalog # A00230) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL4 at approximately 20KD. The expected band size for IL4 is at 17KD.
Specific Publications For Anti-IL4 Antibody Picoband® (A00230)
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4 Customer Q&As for Anti-IL4 Antibody Picoband®
Question
Our lab used your anti-IL4 antibody for ELISA on oocyte last year. I am using human, and I plan to use the antibody for WB next. We are interested in examining oocyte as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
I looked at the website and datasheets of our anti-IL4 antibody and I see that A00230 has been validated on human in both ELISA and WB. Thus A00230 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-16
Question
We have observed staining in human blood. What should we do? Is anti-IL4 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
From literature blood does express IL4. From Uniprot.org, IL4 is expressed in oocyte, blood, among other tissues. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-04
Question
We are currently using anti-IL4 antibody A00230 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-14
Answer
The anti-IL4 antibody (A00230) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-14
Question
Our team were well pleased with the WB result of your anti-IL4 antibody. However we have observed positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL4 supposed to be expressed?
B. Dhar
Verified customer
Asked: 2017-03-03
Answer
From what I have seen in literature, blood does express IL4. Generally IL4 expresses in secreted. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-03-03
