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Product Info Summary
| SKU: | M00652-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F, ICC |
Product info
Product Name
Anti-Ms CD62L Purified Sell Monoclonal Antibody
SKU/Catalog Number
M00652-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms CD62L Purified Sell Monoclonal Antibody (Catalog# M00652-1). Tested in Flow Cytometry, IP, IHC-P, IHC-F, ICC application(s). This antibody reacts with Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms CD62L Purified Sell Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00652-1)
Host
Rat
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
Mel-14
Isotype
Rat IgG2a
Immunogen
C3H/eb mouse B cell lymphoma 38C-13. The rat monoclonal antibody MEL-14 reacts with an extracellular epitope of murine CD62L (L-selectin), a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
Reactive Species
M00652-1 is reactive to Sell in Mouse
Observed Molecular Weight
19 kDa
Background of Sell
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00652-1 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Separation of murine splenocytes stained anti-mouse CD62L (Mel-14) purified antibody (concentration in sample 4 µg/ml, DAR APC, red-filled) from murine splenocytes unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining).
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Flow cytometry surface staining pattern of murine splenocytes stained using anti-mouse CD62L (Mel-14) purified antibody (concentration in sample 4 µg/ml, DAR APC).
Specific Publications For Anti-Ms CD62L Purified Sell Monoclonal Antibody (M00652-1)
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