Rat sL-Selectin PicoKine™ ELISA Kit
SKU | EK0937 |
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Size | 96wells/kit, with removable strips. |
Reactivity | Rat |
Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
Sensitivity | <20pg/ml |
Assay Range | 625pg/ml-40,000pg/ml |
Overview
Product Name | Rat sL-Selectin PicoKine™ ELISA Kit |
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SKU/Catalog Number | EK0937 |
Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
Size | 96wells/kit, with removable strips. |
Description | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat sL-Selectin. 96wells/kit, with removable strips. |
Cite This Product | Rat sL-Selectin PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0937) |
Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)..
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility. |
Sensitivity | <20pg/ml |
Assay Range | 625pg/ml-40,000pg/ml |
Immunogen | Expression system for standard: NSO; Immunogen sequence: W39-N332 |
Reactivity | Rat |
Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
Antibody Clonalities | Capture Antibody | Detection Antibody: monoclonal antibody from mouse|polyclonal antibody from goat |
Assay Details
Kit Components
Catalog number | Description | Quantity |
EK0937-CAP | 96-well plate precoated with anti-Rat SELL antibody | 1 |
EK0937-ST | lyophilized recombinant Rat SELL standard | 40ng/tube |
EK0937-DA | biotinylated anti-Rat SELL antibody | 130ul |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
AR1106-1 | sample diluent buffer | 30ml |
AR1106-2 | antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Rat sL-Selectin PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 20-25min)
Concentration(pg/ml) | 0 | 625 | 2000000 | 4000000 | 200000 | 400000 | 20000 | 40000 |
O.D. | 0.004 | 0.550 | 0.919 | 1.411 | 1.799 | 2.074 | 2.129 | 2.226 |
Intra/Inter Assay Precision
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1474 | 3614 | 24600 | 1588 | 3716 | 23760 |
Standard deviation | 89.91 | 166.24 | 1279.2 | 100.04 | 178.36 | 1663.2 |
CV(%) | 6.1% | 4.6% | 5.2% | 4.8%% | 4.8% | 7% |
Reproducibility
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 1474 | 1304 | 1342 | 1320 | 1360 | 67.18 | 4.9% |
Sample 2 | 3614 | 3452 | 3351 | 3314 | 3432 | 116.19 | 3.3% |
Sample 3 | 24600 | 22997 | 23584 | 22075 | 23314 | 916.82 | 3.9% |
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Target Info
Protein Target Info (Source: Uniprot.org)
Uniprot Id | Q63762 |
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Gene Name | SELL |
Protein Name | L-selectin |
Alternative Names | L-selectin ;Sell ;rCG_45999 ; |
Molecular Weight | 42471 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
Protein Function | Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. . |
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Research Areas | Adaptive Immunity, Cardiovascular, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells, T Cells, T Lymphocytic Lineage *You can search these to find other products in these research areas. |
Background | L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2. |
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Customer Q&As
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
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