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Product Info Summary
| SKU: | M00220-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | Flow Cytometry, IP, IHC-F, ICC |
Product info
Product Name
Anti-Ms CD86 Purified Monoclonal Antibody
SKU/Catalog Number
M00220-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms CD86 Purified Monoclonal Antibody (Catalog# M00220-2). Tested in Flow Cytometry, IP, IHC-F, ICC application(s). This antibody reacts with Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms CD86 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00220-2)
Host
Rat
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
GL-1
Isotype
Rat IgG2a kappa
Immunogen
LPS-activated CBA/Cs mouse splenic B cells. The rat monoclonal antibody GL-1 reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Reactive Species
M00220-2 is reactive to CD86 in Mouse
Observed Molecular Weight
19 kDa
Calculated molecular weight
34.7 kDa
Background of CD86
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00220-2 is guaranteed for Flow Cytometry, IP, IHC-F, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2 μg/ml; positive control: murine splenocytes.
Validation Images & Assay Conditions
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Separation of murine CD86 positive myeloid cells (red-filled) from murine CD86 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine peritoneal fluid cells suspension stained using anti-mouse CD86 (GL-1) purified antibody (concentration in sample 0,6 µg/ml) DAR APC.
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Flow cytometry surface staining pattern of murine peritoneal fluid cells suspension stained using anti-mouse CD86 (GL-1) purified antibody (concentration in sample 0,6 µg/ml) DAR APC.
Specific Publications For Anti-Ms CD86 Purified Monoclonal Antibody (M00220-2)
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