Product Info Summary
SKU: | M00220-2 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Mouse |
Host: | Rat |
Application: | Flow Cytometry, IP, IHC-F, ICC |
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Product info
Product Name
Anti-Ms CD86 Purified Monoclonal Antibody
SKU/Catalog Number
M00220-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms CD86 Purified Monoclonal Antibody (Catalog# M00220-2). Tested in Flow Cytometry, IP, IHC-F, ICC application(s). This antibody reacts with Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms CD86 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00220-2)
Host
Rat
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
GL-1
Isotype
Rat IgG2a kappa
Immunogen
LPS-activated CBA/Cs mouse splenic B cells. The rat monoclonal antibody GL-1 reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00220-2 is reactive to CD86 in Mouse
Applications
M00220-2 is guaranteed for Flow Cytometry, IP, IHC-F, ICC Boster Guarantee
Observed Molecular Weight
19 kDa
Calculated molecular weight
37.682kDa
Background of B7-2/CD86
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2 μg/ml; positive control: murine splenocytes.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry validation of Cd86 using Anti-Ms CD86 Purified Monoclonal Antibody (M00220-2).
Flow cytometry surface staining pattern of murine peritoneal fluid cells suspension stained using anti-mouse CD86 (GL-1) purified antibody (concentration in sample 0Click image to see more details
Figure 2. Flow Cytometry validation of Cd86 using Anti-Ms CD86 Purified Monoclonal Antibody (M00220-2).
Separation of murine CD86 positive myeloid cells (red-filled) from murine CD86 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine peritoneal fluid cells suspension stained using anti-mouse CD86 (GL-1) purified antibody (concentration in sample 0Protein Target Info & Infographic
Gene/Protein Information For CD86 (Source: Uniprot.org, NCBI)
Gene Name
CD86
Full Name
T-lymphocyte activation antigen CD86
Weight
37.682kDa
Alternative Names
Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 CD86 B7-2, B7.2, B70, CD28LG2, LAB72 CD86 molecule T-lymphocyte activation antigen CD86|B-lymphocyte activation antigen B7-2|BU63|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)|CTLA-4 counter-receptor B7.2|FUN-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD86, check out the CD86 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD86: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ms CD86 Purified Monoclonal Antibody (M00220-2)
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