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Product Info Summary
| SKU: | A00057S118-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ER Alpha (Phospho-S118) ESR1 Antibody
SKU/Catalog Number
A00057S118-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ER Alpha (Phospho-S118) ESR1 Antibody catalog # A00057S118-2. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ER Alpha (Phospho-S118) ESR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00057S118-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser118 of human Estrogen Receptor alpha.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00057S118-2 is reactive to ESR1 in Human
Calculated molecular weight
66.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00057S118-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of Estrogen Receptor alpha(phospho S118) on MCF-7 cell lysates using anti- phospho-Estrogen Receptor alpha(S118) antibody at 1/1,000 dilution.
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ICC staining phospho-Estrogen Receptor alpha(S118) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Specific Publications For Anti-ER Alpha (Phospho-S118) ESR1 Antibody (A00057S118-2)
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