Human Clusterin ELISA Kit PicoKine™

Human Clusterin ELISA Kit PicoKine™ (96 Tests). Quantitate Human CLU in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 20pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0914
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Product Name

Human Clusterin ELISA Kit PicoKine™

See all Clusterin/APOJ products

SKU/Catalog Number

EK0914

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Clusterin ELISA Kit PicoKine™ (96 Tests). Quantitate Human CLU in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Clusterin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0914)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D23-R227 (beta) & S228-E449 (alpha)

Sensitivity

<20 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0914 is reactive to CLU in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Application Guarantee

EK0914 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0914-CAP Anti-Human CLU Pre-coated 96-well strip microplate 1
EK0914-ST Human CLU Standard 2 vials, 50 ng/tube
EK0914-DA Human CLU Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0130.0700.1270.2360.4690.9131.8192.780

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Clusterin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)19823789219212148412421708
Standard deviation120.9219.76920.68135.32259.811085.4
CV (%)6.1%5.8%4.2%6.3%%6.3%5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Clusterin/APOJ in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11982199019781909196432.471.6%
Sample 237894380381840214002235.835.8%
Sample 32192120791218961997721146814.813.8%
*number of samples for each test n=16.

Gene/Protein Information For CLU (Source: Uniprot.Org, NCBI)

Uniprot ID

P10909

Gene ID

1191

Gene Name

CLU

Full Name

Clusterin

Weight

52.495kDa

Superfamily

clusterin family

Alternative Names

40; 40, sulfated glycoprotein 2; Aging-associated gene 4 protein; aging-associated protein 4; APOJ; apo-J; Apolipoprotein J; CLI; CLIclusterin (complement lysis inhibitor, SP-40; CLU; Clusterin; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40; Ku70-binding protein 1; KUB1SGP2; MGC24903; NA1/NA2; SGP-2; SP-40; sulfated glycoprotein 2; Testosterone-repressed prostate message 2; testosterone-repressed prostate message 2, apolipoprotein J); TRPM-2; TRPM-2TRPM2 CLU AAG4, APO-J, APOJ, CLI1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, CLU clusterin clusterin|aging-associated protein 4|apolipoprotein J|complement cytolysis inhibitor|complement lysis inhibitor|complement-associated protein SP-40,40|epididymis secretory sperm binding protein|ku70-binding protein 1|sulfated glycoprotein 2|testosterone-repressed prostate message 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CLU, check out the CLU Infographic

CLU infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CLU: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0914 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen QF,Zhang L,Su Q,Zhao Y,Kong B.Clinical importance of serum secreted clusterin in predicting invasive breast cancer and treatment responses.Bioengineered.2020 Dec 26.doi:10.1080/21655979.2020.1868732. Epub ahead of print.PMID:33356806.
Species: Human
EK0914 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Diagnostic and prognostic significance of secretory clusterin expression in patients with hepatocellular carcinoma

Oncogenic secretory clusterin in hepatocellular carcinoma: Expression at early staging and emerging molecular target

Urine clusterin/apolipoprotein J is linked to tubular damage and renal outcomes in patients with type 2 diabetes mellitus

Song J, Zhang Y, Zhang C, Du X, Guo Z, Kuang Y, Wang Y, Wu P, Zou K, Zou L, Lv J0, Wang Q. Sci Rep. 2018 Apr 23;8(1):6394. doi: 10.1038/s41598-018-24748-7. A microfluidic device for studying chemotaxis mechanism of bacterial cancer targeting

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6 Customer Q&As for Human Clusterin ELISA Kit PicoKine™

Question

What is the normal range of clusterin in saliva as well as the dilution factor and detection range of EK0914?

Verified customer

Asked: 2020-12-10

Answer

The range of clusterin in saliva is 82-695ng/ml. Recommended diulution factor is around 1:20. The assay range of standard in the Human Clusterin ELISA Kit PicoKine™ (EK0914) is 0.78ng/ml-50ng/ml. Please estimate the concentration of the target protein in the sample and use an appropriate dilution factor so that the diluted target protein concentration falls in the middle range of O.D. values of the standard curve.

Boster Scientific Support

Answered: 2020-12-10

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

D. Martinez

Verified customer

Asked: 2020-09-23

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-09-23

Question

Q: if the enzyme conjugated Clusterin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Clusterin antigen?

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-05-08

Question

Q: which procedure should I follow in order to thaw whole blood sample for Clusterin ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-02-25

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Clusterin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-02-25

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-07-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-07-13

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Clusterin using the EK0914 Human Clusterin Picokine® ELISA Kit?

N. Harris

Verified customer

Asked: 2018-04-29

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2018-04-29

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