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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.|
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Human Clusterin ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Clusterin ELISA Kit PicoKine™ (96 Tests). Quantitate Human CLU in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human Clusterin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0914)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: D23-R227 (beta) & S228-E449 (alpha)
0.78 ng/ml - 50 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0914 is reactive to CLU in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
EK0914 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0914-CAP||Anti-Human CLU Pre-coated 96-well strip microplate||1|
|EK0914-ST||Human CLU Standard||2 vials, 50 ng/tube|
|EK0914-DA||Human CLU Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Clusterin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Clusterin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Clusterin/APOJ in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CLU (Source: Uniprot.Org, NCBI)
40; 40, sulfated glycoprotein 2; Aging-associated gene 4 protein; aging-associated protein 4; APOJ; apo-J; Apolipoprotein J; CLI; CLIclusterin (complement lysis inhibitor, SP-40; CLU; Clusterin; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40; Ku70-binding protein 1; KUB1SGP2; MGC24903; NA1/NA2; SGP-2; SP-40; sulfated glycoprotein 2; Testosterone-repressed prostate message 2; testosterone-repressed prostate message 2, apolipoprotein J); TRPM-2; TRPM-2TRPM2 CLU AAG4, APO-J, APOJ, CLI1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, CLU clusterin clusterin|aging-associated protein 4|apolipoprotein J|complement cytolysis inhibitor|complement lysis inhibitor|complement-associated protein SP-40,40|epididymis secretory sperm binding protein|ku70-binding protein 1|sulfated glycoprotein 2|testosterone-repressed prostate message 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CLU, check out the CLU Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CLU: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0914 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Apolipoprotein J is a hepatokine regulating muscle glucose metabolism and insulin sensitivity
Chen QF,Zhang L,Su Q,Zhao Y,Kong B.Clinical importance of serum secreted clusterin in predicting invasive breast cancer and treatment responses.Bioengineered.2020 Dec 26.doi:10.1080/21655979.2020.1868732. Epub ahead of print.PMID:33356806.
EK0914 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Diagnostic and prognostic significance of secretory clusterin expression in patients with hepatocellular carcinoma
Oncogenic secretory clusterin in hepatocellular carcinoma: Expression at early staging and emerging molecular target
Urine clusterin/apolipoprotein J is linked to tubular damage and renal outcomes in patients with type 2 diabetes mellitus
Song J, Zhang Y, Zhang C, Du X, Guo Z, Kuang Y, Wang Y, Wu P, Zou K, Zou L, Lv J0, Wang Q. Sci Rep. 2018 Apr 23;8(1):6394. doi: 10.1038/s41598-018-24748-7. A microfluidic device for studying chemotaxis mechanism of bacterial cancer targeting
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