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Product Info Summary
| SKU: | A32480 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MYORG Antibody Picoband®
SKU/Catalog Number
A32480
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MYORG Antibody Picoband® catalog # A32480. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MYORG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32480)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MYORG recombinant protein (Position: Q207-R611). Human MYORG shares 92.9% amino acid (aa) sequence identity with mouse MYORG.
Reactive Species
A32480 is reactive to MYORG in Human, Mouse, Rat
Observed Molecular Weight
87 kDa
Calculated molecular weight
81.1 kDa
Background of MYORG
MYORG promotes myogenesis by activating AKT signaling through the maturation and secretion of IGF2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32480 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, human Hacat whole cell, human Caco-2 whole cell, rat ovary tissue, rat RH35 whole cell, mouse ovary tissue, mouse NIH/3T3 whole cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of MYORG using anti-MYORG antibody (A32480).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat ovary tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse ovary tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYORG antigen affinity purified polyclonal antibody (Catalog # A32480) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYORG at approximately 87 kDa. The expected band size for MYORG is at 81 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-MYORG antibody (A32480).
Overlay histogram showing HepG2 cells stained with A32480 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYORG Antibody (A32480, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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