This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
NCR1 antibody (A01516-2) performs well in ELISA, Flow Cytometry, IHC and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Flow Cytometry analysis of mouse spleen cells using anti-NCR1 antibody (A01516-2).
Overlay histogram showing mouse spleen cells stained with A01516-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NCR1 Antibody (A01516-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank contro
Overlay histogram showing mouse spleen cells stained with A01516-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NCR1 Antibody (A01516-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank contro
Bundle Save
Anti-NCR1 Antibody
NCR1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, Flow Cytometry, IHC |
Tested Applications
NCR1 antibody was positively detected by IHC in:
human tonsil tissue, mouse spleen tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
NCR1 antibody was positively detected by FC in:
mouse spleen cells
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
IHC
IF
ELISA
FC
Human
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Tested
Product Information
Product Name
Anti-NCR1 Antibody
View all NCR1 Antibodies
View all NCR1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NCR1 recombinant protein (Position: A55-R258).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated Molecular Weight
34.5 kDa
Background of NCR1
Natural cytotoxicity triggering receptor 1 is a protein that in humans is encoded by the NCR1 gene. Predicted to be involved in cellular defense response; regulation of natural killer cell mediated cytotoxicity; and signal transduction. Predicted to act upstream of or within defense response to virus and detection of virus. Predicted to be located in cell surface. Predicted to be part of SWI/SNF complex. Predicted to be active in plasma membrane. Biomarker of acquired immunodeficiency syndrome; anogenital venereal wart; hepatitis C; and lymphoproliferative syndrome.
Cite This Product
Anti-NCR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01516-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
89%
Moderate Homology
Rat
67%
Low homology
Dog
67%
Low homology
Sheep
66%
Low homology
Mouse
65%
Low homology
Cow
65%
Low homology
Rabbit
60%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for NCR1 antibody
Flow Cytometry (FC) protocol for NCR1 antibody
Publications
Application:
Species:
Electrospun nanofibrous mats loaded with gemcitabine and cisplatin suppress bladder tumor growth by improving the tumor immune microenvironment
Published: 2024-03-25
Journal: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Application: IF,IHC
Impact Factor: 4.20
Species: Mouse
PMID: 38526656
Review
Have you used Anti-NCR1 Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-NCR1 Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01516-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026