Anti-CD1d Antibody

CD1D antibody

Boster Bio Anti-CD1d Antibody catalog # PA1850. Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1850
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-CD1d Antibody

View all CD1D Antibodies

SKU/Catalog Number

PA1850

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD1d Antibody catalog # PA1850. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD1d Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1850)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CD1d.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1850 is reactive to CD1D in Human

Applications

PA1850 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

31 kDa

Calculated molecular weight

37.717kDa

Background of CD1D

CD1D (CD1d molecule) also known as R3G1, Antigen-presenting glycoprotein CD1d, R3G1, CD1D, is the sole group-2 member of the CD1 family of major histocompatibility (MHC)-like glycoproteins. Expressed on the surface of various human antigen-presenting cells. They are related to the class I MHC molecules, and are involved in the presentation of lipid antigens to T cells. It is the only member of the group 2 CD1 molecules. CD1d presented lipid antigens activate a special class of T cells, known as Natural Killer T (NKT) cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines, typically represented by interferon-gamma and IL-4 production. Jayawardena-Wolf et al. described 2 different pathways of Cd1d trafficking to endosomal compartments in mouse cells. A tyrosine-based motif governs recycling between the plasma membrane and the endosome, while Cd1d associates, like major histocompatibility complex class II antigens, with the invariant chain, or II, in the endoplasmic reticulum. Both pathways enhance antigen presentation to Cd1d-restricted natural killer T cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD1D (Source: Uniprot.org, NCBI)

Gene Name

CD1D

Full Name

Antigen-presenting glycoprotein CD1d

Weight

37.717kDa

Alternative Names

antigen-presenting glycoprotein CD1d; CD1A; CD1d antigen; CD1D antigen, d polypeptide; CD1d molecule; CD1d; differentiation antigen CD1-alpha-3; HMC class I antigen-like glycoprotein CD1D; MGC34622; R3; R3G1; T-cell surface glycoprotein CD1d; thymocyte antigen CD1D CD1D CD1A, R3, R3G1 CD1d molecule antigen-presenting glycoprotein CD1d|CD1D antigen, d polypeptide|HMC class I antigen-like glycoprotein CD1D|T-cell surface glycoprotein CD1d|differentiation antigen CD1-alpha-3|thymocyte antigen CD1D

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD1D, check out the CD1D Infographic

CD1D infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-CD1d Antibody

Question

Would anti-CD1d antibody PA1850 work on feline IHC with pancreas?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

Our lab technicians have not tested anti-CD1d antibody PA1850 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CD1d antibody PA1850 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline pancreas in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-10

Question

Is a blocking peptide available for product anti-CD1d antibody (PA1850)?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

We do provide the blocking peptide for product anti-CD1d antibody (PA1850). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-04

Question

Does PA1850 anti-CD1d antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

E. Carter

Verified customer

Asked: 2019-09-10

Answer

As indicated on the product datasheet, PA1850 anti-CD1d antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-10

Question

I was wanting to use your anti-CD1d antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2019-04-19

Answer

You can see on the product datasheet, PA1850 anti-CD1d antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-04-19

Question

We are currently using anti-CD1d antibody PA1850 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-28

Answer

The anti-CD1d antibody (PA1850) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-28

Question

Is this PA1850 anti-CD1d antibody reactive to the isotypes of CD1D?

O. Edwards

Verified customer

Asked: 2013-07-12

Answer

The immunogen of PA1850 anti-CD1d antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CD1d(76-92aa FSDQQWETLQHIFRVYR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-07-12

Order DetailsPrice
PA1850

100μg

$370
PA1850-10ug

10μg sample (liquid)

$99
PA1850-Biotin

100 μg Biotin conjugated

$570
PA1850-Cy3

100 μg Cy3 conjugated

$570
PA1850-Dylight488

100 μg Dylight488 conjugated

$570
PA1850-Dylight550

100 μg Dylight550 conjugated

$570
PA1850-Dylight594

100 μg Dylight594 conjugated

$570
PA1850-FITC

100 μg FITC conjugated

$570
PA1850-HRP

100 μg HRP conjugated

$570
PA1850-APC

100 μg APC conjugated

$670
PA1850-PE

100 μg PE conjugated

$670
PA1850-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1850
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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