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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human MICB ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human MICB ELISA Kit PicoKine™ (96 Tests). Quantitate Human MICB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MICB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0963)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A23-G298
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0963 is reactive to MICB in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0963 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MICB
MHC class I polypeptide-related sequence B, also called MICB is a protein that in humans is encoded by the MICB gene. The MICB gene is mapped to 6p21.33. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. It seems to have no role in antigen presentation. This gene acts as a stress-induced self-antigen that is recognized by gamma delta T cells. It is the ligand for the KLRK1/NKG2D receptor and binds to KLRK1 leads to cell lysis.
|EK0963-CAP||Anti-Human MICB Pre-coated 96-well strip microplate||1|
|EK0963-ST||Human MICB Standard||2 vials, 10 ng/tube|
|EK0963-DA||Human MICB Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human MICB PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MICB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MICB in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MICB (Source: Uniprot.Org, NCBI)
MHC class I polypeptide-related sequence B
MHC class I family
MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence B; MICB; MIC-B; PERB11.2MHC class I-like molecule PERB11.2-IMX; stress inducible class I homolog MICB PERB11.2 MHC class I polypeptide-related sequence B MHC class I polypeptide-related sequence B|MHC class I antigen-related protein B|MHC class I chain-related protein B|MHC class I mic-B antigen|MHC class I-like molecule PERB11.2-IMX|stress inducible class I homolog*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MICB, check out the MICB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MICB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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