Anti-RORC phospho S203 Antibody

RORC antibody

Boster Bio Anti-RORC phospho S203 Antibody (Catalog # A00444). Tested in ELISA, WB applications. This antibody reacts with Mouse. Cited in 1 publication(s).

Product Info Summary

SKU: A00444
Size: 100ug
Reactive Species: Mouse
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-RORC phospho S203 Antibody

View all RORC Antibodies

SKU/Catalog Number

A00444

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-RORC phospho S203 Antibody (Catalog # A00444). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. This product is stable at 4°C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-RORC phospho S203 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00444)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Affinity purified Anti-receptor ROR gamma pS203 antibody was prepared from whole rabbit serum produced by repeated immunizations with an internal synthetic peptide phosphorylated at the serine 203 position of mouse receptor ROR gamma protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00444 is reactive to RORC in Mouse

Applications

A00444 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

58.195kDa

Background of RORC

Anti-Nuclear receptor ROR gamma pS203 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Nuclear receptor ROR gamma pS203 antibody detects mouse receptor ROR gamma phosphorylated at the serine 203 position. RAR-related orphan receptor gamma is a member of the nuclear receptor family of transcription factors. (ROR-gamma) is a key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. ROR-gamma regulates the circadian expression of clock genes such as CRY1, ARNTL/BMAL1 and NR1D1 in peripheral tissues and in a tissue-selective manner. It is also involved in the regulation of the rhythmic expression of genes involved in glucose and lipid metabolism and is a negative regulator of adipocyte differentiation. It controls adipogenesis as well as adipocyte size and modulates insulin sensitivity in obesity. Isoform 2, ROR-gamma is essential for lymphoid organogenesis, in particular lymph nodes and Peyer's patches. ROR-gamma also plays an important regulatory role in thymopoiesis, and in inhibiting apoptosis of undifferentiated T cells. Anti-nuclear receptor ROR gamma pS203 is ideal for researcher's interested in autoimmune diseases such as psoriasis and rheumatoid arthritis, circadian rhythms, and immune system disorders.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 5 µg/ml
WB: 1 ug/mL

Validation Images & Assay Conditions

Gene/Protein Information For RORC (Source: Uniprot.org, NCBI)

Gene Name

RORC

Full Name

Nuclear receptor ROR-gamma

Weight

58.195kDa

Superfamily

nuclear hormone receptor family

Alternative Names

ROR gamma; MGC129539; NR1F3; Nuclear receptor ROR gamma; Nuclear receptor ROR-gamma; Nuclear receptor RZR gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-Related Orphan Nuclear Receptor Variant 2; RAR-related orphan receptor C; RAR-related orphan receptor gamma; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; Retinoid-related orphan receptor-gamma; ROR gamma; RORC; RORG; RORGMGC129539; RZRG; RZRGRZR-GAMMA; TOR RORC IMD42, NR1F3, RORG, RZR-GAMMA, RZRG, TOR RAR related orphan receptor C nuclear receptor ROR-gamma|RAR-related orphan nuclear receptor variant 2|nuclear receptor RZR-gamma|nuclear receptor subfamily 1 group F member 3|retinoic acid-binding receptor gamma|retinoid-related orphan receptor gamma

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RORC, check out the RORC Infographic

RORC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RORC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00444 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Impaired TNF/TNFR2 signaling enhances Th2 and Th17 polarization and aggravates allergic airway inflammation

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5 Customer Q&As for Anti-RORC phospho S203 Antibody

Question

I see that the anti-Nuclear receptor ROR gamma pS203 antibody A00444 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-11

Question

I was wanting to use your anti-Nuclear receptor ROR gamma pS203 antibody for WB for mouse lymph node on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lymph node identification?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

You can see on the product datasheet, A00444 anti-Nuclear receptor ROR gamma pS203 antibody has been tested for ELISA, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse lymph node in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-04

Question

We are currently using anti-Nuclear receptor ROR gamma pS203 antibody A00444 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-29

Answer

The anti-Nuclear receptor ROR gamma pS203 antibody (A00444) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-29

Question

Please see the WB image, lot number and protocol we used for lymph node using anti-Nuclear receptor ROR gamma pS203 antibody A00444. Please let me know if you require anything else.

N. Taylor

Verified customer

Asked: 2017-12-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-12-25

Question

I have a question about product A00444, anti-Nuclear receptor ROR gamma pS203 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Patel

Verified customer

Asked: 2016-06-30

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00444 anti-Nuclear receptor ROR gamma pS203 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-06-30

Order DetailsPrice
A00444

100 μg

$599

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