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Product Info Summary
| SKU: | A00122 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-PARP PARP1 Antibody
SKU/Catalog Number
A00122
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PARP PARP1 Antibody catalog # A00122. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PARP PARP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00122)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic peptide of human PARP1(NP_001609.2).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00122 is reactive to PARP1 in Human, Mouse, Rat
Calculated molecular weight
113.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00122 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:3000
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:3000 dilution.HeLa cells were treated by staurosporine for 3 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
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Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.
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Confocal immunofluorescence analysis of U-2 OS cells using PARP1 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
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Expression of P-gp and Cle-PARP in tumour tissue form mice after treatment with different formulations: 1. Saline; 2. DOX solution; 3. F-pHSM; 4. F-pHSM/CUR; 5. F-pHSM-L61; 6. F-pHSM-L61/CUR; 7. F-pHSM/CUR/DOX; 8. F-pHSM-L61/DOX; 9. F-pHSM-L61/CUR/DOX.
Index in PubMed under a CC BY license. PMID: 28195164
Specific Publications For Anti-PARP PARP1 Antibody (A00122)
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